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组织学分析实验-小鼠骨骼标本制作及双色染色
简介小鼠骨骼标本制作及双色染色是先将小鼠骨骼以外的身体成分消化掉，再通过硬骨和软骨的不同特征将其分别用对比鲜明的色彩进行整体染色，可用于直观地表现整体骨骼的发育
2024-12-24 130 -
新生儿胎粪吸入综合征动物模型的建立
简介胎粪吸入通过不均匀的气道阻塞、肺表面活性物质 (PS) 功能障碍、化学性肺炎、肺动脉高压等 4 种主要效应引起通气一血流灌注比例失衡，导致缺氧。原理模拟胎儿
2024-12-24 25 -
新生儿胆红素脑病动物模型的建立
简介新生儿发生严重高胆红素血症时，脂溶性的未结合胆红素通过血脑屏障，沉积于基底神经核、丘脑、小脑、延髓、大脑皮质、脊髓等部位，进入神经细胞，使其变性、死亡，以致
2024-12-24 26 -
大鼠嵌合体制备技术-ES细胞显微注射法制作嵌合体大鼠
简介嵌合体（chimera）在遗传学上用于指不同遗传性状嵌合或混杂表现的个体；在免疫学上的涵义则指一个机体身上有两种或两种以上染色体组成不同的细胞系同时存在，彼
2024-12-24 28 -
大鼠嵌合体制备技术-聚集法制作嵌合体大鼠
简介嵌合体（chimera）在遗传学上用于指不同遗传性状嵌合或混杂表现的个体；在免疫学上的涵义则指一个机体身上有两种或两种以上染色体组成不同的细胞系同时存在，彼
2024-12-24 26 -
新生儿缺氧缺血性脑病动物模型的建立
简介建立 HIBD 动物模型的经典方法是 Rice-Vannucci 模型，大鼠脑的血液供应与人类相似，颈内动脉和椎动脉在大脑底部形成 Willis 环。在研究
2024-12-24 34 -
Cell Labeling and Gene Misexpression by Electroporation
Recent advances in molecular biology have led to the identification of several g
2024-12-24 11 -
Methods to Detect Patterns of Cell Death in Drosophila
There are many times during Drosophila development or in the adult when cell dea
2024-12-24 13 -
Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues
Fluorescent in situ hybridization is the standard method for visualizing the spa
2024-12-24 22 -
The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes
Biophysical measurements of multidrug transporters in vitro can often be of limi
2024-12-24 23 -
Assaying Phospholipase A2 Activity
Mammalian cells contain many structurally and functionally diverse phospholipase
2024-12-24 15 -
Analyzing p53 Regulated DNA Damage Checkpoints by Flow Cytometry
The most critical feature of the cellular response to DNA damage is the ability
2024-12-24 11 -
Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes
The cytogenetic analysis of single cells, such as oocytes and polar bodies, is e
2024-12-24 12 -
Permeability of Differentiated Human Urothelium In Vitro
The urothelium plays a critical role in the bladder as a permeability barrier to
2024-12-24 8 -
The Use of Permeabilized Cell Systems to Study Nuclear Transport
Nucleocytoplasmic transport occurs through nuclear pore complexes (NPC), macromo
2024-12-24 11
