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单盐毒害作用及离子间的对抗作用实验
原理任何植物如果培养在单一种盐溶液中,不久即呈现不正常状态,最后导致死亡。这种单盐毒害现象,即使在浓度很低,而且是植物所必需的元素的单盐溶液中也会发生。尤其是阳
2025-01-04 78 -
离体法植物体内硝酸还原酶活性的测定实验
原理硝酸还原酶(NR)催化植物体内的硝酸盐还原为亚硝酸盐,产生的亚硝酸盐与对-氨基苯磺酸(或对-氨基苯磺胺)及α-萘胺(或萘基乙烯二胺)在酸性条件下定量生成红色
2025-01-04 39 -
活体法检测物体内硝酸还原酶活性实验
步骤一、材料仪器设备及试剂1. 材料:水稻或小麦的叶片、幼穗等。2. 仪器设备:真空抽气泵;真空干燥器;小烧杯;玻璃瓶塞;其它用具同离体法。3. 试剂及配制:亚
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植物体内硝态氮含量的测定实验
原理在强酸条件下NO3-与水杨酸反应,生成硝基水杨酸。生成的硝基水杨酸在碱性条件下(pH>12)呈黄色,在一定范围内,其颜色的深浅与含量成正比,可直接用分光光度
2025-01-04 50 -
叶绿素的理化性质测定实验
原理叶绿素是一种二羧酸的酯,可与碱起皂化作用,产生的盐能溶于水,可用此法将叶绿素与类胡萝卜素分开。叶绿素与类胡萝卜素都具有共轭双键,在可见光区表现出一定的吸收光
2025-01-04 40 -
水稻种子萌发对氧的需要实验
原理水稻系淹水植物,对缺氧环境有一定的适应能力,但适应方式主要是借助发达的通气组织使根系得到从地上部运来的氧气。水稻种子能在氧气不足的水中萌发,这是一般作物如小
2025-01-04 41 -
Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb
The ability to produce highly purified populations of individual cell types is c
2025-01-03 42 -
An Overview of HLA Typing for Hematopoietic Stem Cell Transplantation
Selection of a related or unrelated haematopoietic stem cell donor for a patient
2025-01-03 35 -
Identification of Mutations in the Kir6.2 Subunit of the KATP Channel
The beta-cell ATP-sensitive potassium channel is a key component of stimulus —se
2025-01-03 35 -
Detection by TUNEL labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling by Boehringer Mannhei
2025-01-03 34 -
Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells
The popularity of nonhematopoietic, adult tissue-derived stem and progenitor cel
2025-01-03 30 -
Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells
This chapter describes the analysis of long- and short-lived plasma cells on tis
2025-01-03 27 -
In Situ Hybridization for Metalloproteinases and Their Inhibitors
The imminent completion of the human and mouse genome projects will reveal all m
2025-01-03 34 -
Profiling the Metabolic Signature of Senescence
Aging is a complex process, which involves changes in different cellular functio
2025-01-03 33 -
COPⅡ被膜小泡(COPⅡ coated vesicle)
由外被蛋白Ⅱ(coat protein Ⅱ, COP Ⅱ )包裹的小泡。外被蛋白是一个大的复合体,称为外被体(coatomer)。这种类型的小泡介导非选择性运输
2025-01-03 25