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  • Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture

    Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture

    Human bone marrow (BM) contains a population of non-hematopoietic stem cells als

    2025-01-22 77
  • Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation

    Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation

    Epigenetic modifications on the DNA sequence (DNA methylation) or on chromatin-a

    2025-01-22 60
  • 关于乳鼠心肌细胞培养的一点收获

    关于乳鼠心肌细胞培养的一点收获

    在园子里浏览了很多次,发现关于乳鼠心肌细胞培养的资料还是几年前的比较多,不知道是不是这几年已经做得很少了。我是今年刚开始做的,发现虽然说方法已经很成熟了,但是做

    2025-01-22 61
  • Quantitation of Tacrolimus in Whole Blood Using High Performance Liquid Chromatography-Tandem Mass S

    Quantitation of Tacrolimus in Whole Blood Using High Performance Liquid Chromatography-Tandem Mass S

    We describe a multiple reaction monitoring positive ion HPLC/tandem mass spectro

    2025-01-22 52
  • In Vivo Imaging of Oligonucleotidic Aptamers

    In Vivo Imaging of Oligonucleotidic Aptamers

    In this chapter we present the methods developed in our laboratory for in vivo i

    2025-01-22 48
  • Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes

    Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes

    In addition to their metabolic function of storing triglycerides and releasing f

    2025-01-22 48
  • Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

    Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

    The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the r

    2025-01-22 49
  • Neural Transplantation and Stem Cells

    Neural Transplantation and Stem Cells

    Recent results have raised important questions on our ability to amplify stem ce

    2025-01-22 45
  • Determination of Gene Expression Patterns by In Situ Hybridization in Sections

    Determination of Gene Expression Patterns by In Situ Hybridization in Sections

    In recent years, in situ RNA hybridization technique has found a widespread appl

    2025-01-22 34
  • Gene Modification with Hapaxoterministic Restriction Enzymes: Easing the Way

    Gene Modification with Hapaxoterministic Restriction Enzymes: Easing the Way

    Subcloning can be extraordinarily easy or extremely difficult. The simplest case

    2025-01-22 36
  • Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix

    Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix

    Mesenchymal stem cells (MSCs) have huge potential to be used in cell therapies b

    2025-01-22 38
  • Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells

    Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells

    Compared to eutherian mammals, birds retain a primitive form of epiblast develop

    2025-01-22 33
  • Isolating Aptamers Using Capillary ElectrophoresisSELEX (CESELEX)

    Isolating Aptamers Using Capillary ElectrophoresisSELEX (CESELEX)

    SELEX (systematic evolution of ligands by exponential enrichment) is a process f

    2025-01-22 41
  • Expansion of Pluripotent Stem Cells in Defined, Xeno-Free Culture System

    Expansion of Pluripotent Stem Cells in Defined, Xeno-Free Culture System

    The hallmark of pluripotent stem cells is their nearly unlimited self-renewal ca

    2025-01-22 42
  • Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells

    Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells

    During malignant transformation, cells can increase their ploidy and hence becom

    2025-01-22 41