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Morphometric Evaluation of the Spermatogonial Stem Cell Distribution and Niche in Vertebrates
Morphometry is a classical quantitative method often used in biology to provide
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The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embr
During embryonic development, primitive ectoderm forms three primary germ layers
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Intrathymic Injection for Analysis of T-Cell Progenitor Activity
Within our field, improvement in fluorescence-activated cell sorting (FACS) and
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Long-Term Culture-Initiating Cell Assays for Human and Murine Cells
In normal adults, the majority of primitive hematopoietic cells are concentrated
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Analysis of Phosphoinositide Dynamics During Phagocytosis Using Genetically Encoded Fluorescent Bios
Phosphoinositide signaling is essential for successful phagocytosis. Phosphoinos
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High-Throughput Screens Based on NAD(P)H Depletion
Screening conditions should simulate the final application as closely as possibl
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Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescentl
Cells communicate via endo- and exocytosis with their environment and neighborin
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细胞转染操作步骤
相关专题转染 ，是将外源性基因导入细胞内的一种专门技术。随着基因与蛋白功能研究的深入，转染目前已成为实验室工作中经常涉及的基本方法。转染大致可分为物理介导、化学
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Probing RNA-Protein Interactions by Psoralen Photocrosslinking
RNA molecules can fold into extensive structures containing regions of double-st
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Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction
Investigation of the in vivo regulation of gene expression in human subcutaneous
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Peptidomics: Identification of Pathogenic and Marker Peptides
Recent years have seen great advances in mass spectrometry and proteomics, the s
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Application of PepFect Peptides for the Delivery of Splice-Correcting Oligonucleotides
One oligonucleotide-based approach that appear very promising for the treatment
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Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver
Oval cells and small hepatocytes (SHs) are well known as hepatic stem/progenitor
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内含信号序列(internal signal sequence)
内含信号序列又称内含信号肽(internal signal peptides),它不位于N-末端,但具信号序列的作用,故称为内含信号序列。它可作为蛋白质共翻译转
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In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods
In this chapter we describe the use of cRNA (riboprobes) in the detection of gen
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