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Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry
Inhaled particles can cause a spectrum of responses ranging from simple clearanc
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Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS)
3D chemical microscopy is one of the emerging applications of secondary ion mass
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Dictyostelium Slug Phototaxis
Dictyostelium slugs are able to respond to environmental stimuli in an extremely
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Following the Fate of Neural Progenitors by Homotopic/Homochronic Grafts in Xenopus Embryos
The neural plate consists of neuroepithelial cells that serve as progenitors for
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The Use of Selective Enrichment for the Isolation of Species-Specific DNA Probes for Insects
The identification of insects and other invertebrates is often extremely difficu
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Protein Kinase-A Directed Antisense Therapy of Tumor Growth In Vivo: The Antisense Effects Outlast A
Standard chemotherapy for cancer usually is accompanied by systemic tox icity, r
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Immobilization of Enzymes on Monofunctional and Heterofunctional Epoxy-Activated Supports
The immobilization of proteins on epoxy activated supports is discussed in this
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Isolation of Human and Mouse Hematopoietic Stem Cells
Hematopoietic stem cells (HSC) are rare with estimated frequencies of 1 in 10,00
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Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System
Photoaffinity labeling is the process of covalently crosslinking a photoactive l
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Cloning of Hybridomas
Hybrid clones will appear within 2–3 wk after fusion (see Chapter 46 ). It is of
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植物体内抗坏血酸氧化酶活性的测定实验
原理抗坏血酸氧化酶在有氧情况下,能氧化抗坏血酸成脱氢抗坏血酸,同时促进其氢与空气中的氧结合成水。抗坏血酸氧化酶的测定是在该酶的最适pH及适宜的温度下,向反应瓶中
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植物体内多酚氧化酶活性的测定实验
原理多酚氧化酶是一种含铜的氧化酶,在有氧的条件下,能使一元酚和二元酚氧化产生醌。用分光光度法在525nm波长下测其吸光度,即可计算出多酚氧化酶的活力和比活性。反
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植物蔗糖含量的测定实验
原理植物体内可溶性糖包括还原糖和非还原糖(主要是蔗糖)。因此测定蔗糖时,须先用盐酸将其水解为还原糖,再测定水解后的总还原糖含量,然后从总还原糖含量减去水解前还原
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植物体内可溶性蛋白和非可溶性蛋白含量的测定实验
原理植物材料经Tris-HCl缓冲液研磨、离心后,可溶性蛋白质溶于上清液中,非可溶性蛋白则存在于沉淀部分。将沉淀用碱水解,则会得到非可溶性蛋白的提取液。蛋白质提
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生长素对绿豆苗发根的影响实验
原理生长素不但能促进细胞的伸长与长大,而且能促进细胞的分裂与分化,用适宜浓度的生长素处理绿豆苗的下胚轴,可诱导不定根的发生。在一定浓度范围内,发生的根数与生长素
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