-
小鼠肥大细胞的形态学观察实验
原理肥大细胞的胞质中充满许多粗大的水溶性、嗜碱性和异染性的颗粒,颗粒中含有多巴胺,组胺,肝素。5-羟色胺等活性物质。这些物质中含有多硫酸脂和硫酸黏多糖类,能与异
2024-12-27 106 -
小鼠睾丸活精子抑制观察实验
原理睾丸曲细精管中的精原细胞,在垂体促性腺激素的作用下,经2-3次有丝分裂后,部分细胞演变成初级精母细胞。初级精母细胞又经两次成熟分裂后,形成精子细胞,但尚未成
2024-12-27 89 -
细胞的吞噬活动与矽肺发生的形态学观察实验
原理高等动物体内存在着具有防御功能的吞噬细胞系,其中粒细胞和单核细胞的吞噬能力最强,故称吞噬细胞,这些细胞随血液进入组织后逐渐演变成巨噬细胞。 当机体受到异物入
2024-12-27 77 -
早期胚胎培养及形态学观察实验
原理鸡胚胎发育可分为两个阶段:成蛋阶段的发育与成雏阶段的发育。1、胚胎在卵形成过程中的发育即母体内的发育,是成蛋阶段的发育。这个阶段的发育过程是:受精卵卵裂囊胚
2024-12-27 110 -
静脉内皮细胞对组胺反应的形态学实验
原理通过实验可以见证,当人体接触到过敏原时,巨噬细胞—B淋巴细胞—浆细胞的免疫应答过程中产生IGE,肥大细胞释放组胺,引起了血管扩张和通透性增强,称为:“速发短
2024-12-27 97 -
The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Co
Adhesive interactions of cells with blood vessel walls under flow conditions are
2024-12-27 55 -
Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identifica
The nuclear envelope (NE) is a double membrane system that is both a part of the
2024-12-27 126 -
核酶(ribozyme)
核酶一词用于描述具有催化活性的RNA, 即化学本质是核糖核酸(RNA), 却具有酶的催化功能。核酶的作用底物可以是不同的分子, 有些作用底物就是同一RNA分子中
2024-12-27 54 -
细胞培养常见问题及回答
细胞培养常见问题及回答 如何选用特殊细胞系培养基? 培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很容易生长。总之,
2024-12-27 146 -
Generation and Establishment of Murine Adherent Cell Lines
We describe a method to derive cell lines and clones from cells of the murine mi
2024-12-27 53 -
Analysis of Myristoylated and Palmitoylated Src Family Proteins
Several hundred viral and cellular proteins have been shown to be covalently mod
2024-12-27 52 -
Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development
Wnts play a central role in the development of many cells and tissue types in al
2024-12-27 68 -
Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies
Mammary reconstitution assays can be used to measure the stem cell frequency wit
2024-12-27 51 -
In Vivo and In Vitro Models of Mammalian Angiogenesis
Angiogenesis is a complex process involving the organization of proliferating en
2024-12-27 66 -
Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans
A strategy for determining the structure of a glycosaminoglycan (GAG) can be div
2024-12-27 40