-
蛋白复合体直接酶消化法Direct Enzymatic Digestion of Protein Complexes
Direct Enzymatic Digestion of Protein ComplexesSherry Niessen, Ian McLeod and Jo
2024-12-04 41 -
Home-made Taq Polymerase Purification
Home-made Taq Polymerase PurificationI also have the bacteria containing the clo
2024-12-04 48 -
ELISA FOR DETECTING SECRETED ICAM-1
PurposeMaterialsNUNC-Immuno Plate IIF.Horseradish Peroxidase-Streptavidin (Zymed
2024-12-04 39 -
WESTERN BLOTTING USING CHEMILUMINESCENCE
REAGENTS ECL Western blotting kit (Amersham Life Science; cat# RPN2108): contai
2024-12-04 57 -
Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions In
2024-12-04 44 -
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
IntroductionThis is an adaptation of the published procedure for RNAse treatment
2024-12-04 52 -
Western Blot Protocols
Western Blot MaterialsSemi-dry Transfer ApparatusBiorad, Cat# 170-3940, or equiv
2024-12-04 50 -
Protocol for anti-HA antibody Western Blotting
1) Run gel (in 1:10 running/transfer buffer(10x) and H2O for a total of 1litre)
2024-12-04 43 -
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarso
2024-12-04 50 -
运用肽库筛选磷酸化激酶motif方法 Screening Kinase Phosphorylation Motifs Using Peptide Libraries
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke an
2024-12-04 49 -
Synthesis and Probing of Membrane-bound Peptide Arrays
Synthesis and Probing of Membrane-bound Peptide Arrays Ronald Fra
2024-12-04 47 -
氰化高铁血红蛋白测定法
[目的与原理]应用氰化高铁血红蛋白（HiCN）法，测定不同鱼类血红蛋白含量。血红蛋白(Hb)被高铁氰化钾[K 3 Fe(CN) 6 ]氧化为高铁血红蛋白（Hi）
2024-12-04 61 -
动物血中超氧化物歧化酶的提取
[原理] l969年，McCord和Fridovich第一次从牛血中提纯到超氧化物岐化酶。 自然界中SOD分布极广，其含量随生物体的不同而不同，即使同一种生物的
2024-12-04 53 -
外源基因的诱导表达
1．目的了解外源基因在原核细胞中表达的特点和方法。2．原理外源基因克隆在含有lac启动子的表达系统中。先让宿主菌生长，lac I产生的阻遏蛋白与lac操纵基因结
2024-12-04 63 -
15种蛋白质单晶培养的方法
15种蛋白质单晶培养的方法： （1）大量养晶法（bulk crystallization）：若急着养出单晶，则将纯化之蛋白质溶液加入固体盐类或饱和盐液，直到溶液
2024-12-04 221
