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几种分子筛凝胶及应用
做纯化的人可能经常接触到sephadex，BIO-Gel，sephacryl s-,sepharose BIo-Gel等几种填料，有时会混淆在一起，看到一个贴子
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Column Buffers: CREB
Bases Buffer is Buffer C (as per Schroter)(as per SRF)pg.2720mM Hepes pH 7.90.1%
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Purification of TFIIA from E. coli
Linda Warfield,Hahn Lab,2003Volumes given are for 2L of cells for each subunit (
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Procedure to purify protein from cells
This procedure is used to break cells that contain a protein to be purified.This
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Preparation of Dialysis tubing
1)Boil the tubing on a sitr plate (preferably in the hood)in a 4L volume of 2% (
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Myosin Light Chain Preparation
To be done the day before:1.Put meat grinder and accessories in the cold room.2.
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Purification of TFIIIC
SummaryWCE Extracts are prepared from strain SHY282 (TFC4-Flag-EE (HpaI)).TFIIIC
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Preparation of Affinity Column制备亲和层析柱【UCSF】
1.Add 222.2μl 1M MOPS,pH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final
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Crystallization Trials
MaterialsMilipore filter type HA 0.45 micronCulture plates (Linbro model 76-033-
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Preparation of Cell Lysate
1. Wash adherent cells twice in the dish or flask with ice-cold PBS and drain of
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Purification of recombinant sBRF M166L
J. Movius, K, Coachman, and S. Hahn (Hahn Lab)Induction of BRF in bacteria and p
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Preparation of Cell Lysate
1.Wash adherent cells twice in the dish or flask with ice-cold PBS and drain off
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Purification of dnEBNA-1/Soft from E. coli BL21 LysS
Inoculate 2ml of 5ml o/n culture of either p3133 (empty vector pET11a)or p3134 (
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Rapid Yeast Protein Prep for SDS PAGE and Western
Sample Buffer:10 ml0.06M Tris-HCl, pH 6.80.6 ml 1M Tris 6.810% (v/v) glycerol2 m
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Large scale nuclear extract preparat
Large scale nuclear extract preparat关键词： nuclear extract preparat来源： 互联网Hattoti&
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