-
Purifying Protein from Inclusion Bodies
Buffer ABuffer B50 mM tris-HCl, pH 8.020 mM Na2 HPO4 , pH 7.25 mM EDTA20 mM NaCl
2024-11-25 26 -
Preparation of protein extracts for western blot
1.Grow cells to mid-log (OD600 less or equal to 1.0).2.Harvest about 5 OD's
2024-11-25 57 -
Urea Lysis Protocol
9M Urea,2.5mM EDTA,2.5mM EGTA,1% DTE,4% CHAPSmake 10ml and aliquot 10x1ml,freeze
2024-11-25 30 -
TCA protein precipitation
Kyle Coachman/Linda Warfield/Steve HahnLast modified 5/13/02To concentrate prote
2024-11-25 54 -
Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale)Hahn Lab (adapted from J.Leatherwood,Ptashne
2024-11-25 26 -
Purification of GST fusion proteins in E.coli GST融合蛋白纯化，方法二
GST Protein Prep.Ver.21)Grow 50ml of culture in LB or TB antibiotic o/n at 37℃ s
2024-11-25 53 -
Yeast protein prep for SDS PAGE and western (rapid)
Horvath and Riezman,Yeast,1994; Gottschling LabSample Buffer:10 ml0.06M Tris-HCl
2024-11-25 26 -
Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura
2024-11-25 21 -
Purification of Calmodulin from Bovine Brain
References: Watterson et al.(1980)J Biol Chem 255:962-75.Charbonneau et al.(1979
2024-11-25 26 -
离子交换层析介质的技术数据
关于离子交换层析实验的介绍，对于常用到的层析介质的一些基本技术数据罗列如下： 离子交换介质名称最高载量颗粒大小（μm ）特性/ 应用pH 稳定性工作耐压（MPa
2024-11-25 27 -
Isolation of Protein from Tissue
Place the tissues on labeled aluminum foil and immediately place in dry ice.It i
2024-11-25 29 -
Purification of Myosin Light Chain Kinase
Solutions1.1 M Acetic Acid2.Buffer A Homogenization 10 mM MOPS- 40 ml of 2M stoc
2024-11-25 24 -
Bacterial Protein Extraction (mini-scale)
Bacterial Protein Extraction (mini-scale)Using B-PerB-Per (Pierce,Cat number: 78
2024-11-25 51 -
Fusion Protein Isolation 融合蛋白分离纯化
1.Start a 5 ml overnight culture from a frozen stock (in the -70℃)in the followi
2024-11-25 24 -
Preparation of Tonsil Lysate
PurpseThe prtcl describes hw t prepare human tnsil lysate fr use in purificatin
2024-11-25 64
