-
基因突变的分子机制
基因突变包括DNA上碱基对的改变和碱基对的插入或缺失。1．碱基替换碱基替换是指一种碱基被另一种碱基所替代。当DNA分子中一个嘌呤被另一个嘌呤、或者一个嘧啶被另一
2024-10-22 20 -
DNA重组实验方法
实验原理DNA重组是将外源DNA与载体分子连接，这样重新组合的DNA叫做重组体或重组子。DNA重组的方法主要有粘端连接法和平端连接法。 重组的DNA分子是在DN
2024-10-22 24 -
粘性末端的连接
1) The ligation mixture contains the following:vector DNA (~100 ng) insert DNA (
2024-10-22 16 -
CRISPR技术分享
近年来，CRISPR技术掀起了基因组编辑的热潮。短短一年间，CRISPR技术已登上了数个知名榜单，包括Science的十大科学突破，Nature Methods
2024-10-22 14 -
T-RFLP简介
T-RFLP中文可翻译为：末端限制性片段长度多样性，又可以称为16sRNA基因的末端限制性片段分析技术。是一种新兴的研究微生物多态性的分子生物学方法，日亦受到研
2024-10-22 16 -
CsClPrepofPlasmidDNA
This is a standard large scale prep.for plasmid DNA which gives a yield of 0.5-1
2024-10-22 14 -
Isolation of DNA from Acrylamide GELS
1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is
2024-10-22 14 -
DNAandRNAEXTRACTIONS
1) Take one medium sized leaf or half a large leaf (5 to 20 cm^2), weigh and fre
2024-10-22 13 -
CAT ASSAY (liquid phase)
1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flasks, shake & pour into
2024-10-22 15 -
Phenol chloro form Extraction of DNA
Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturat
2024-10-22 13 -
Tail Preps: DNA Isolation From
NOTE: THIS IS FINE FOR SOUTHERNS, BUT NOT FOR SCREENING BY PCR.(from Ruixia, 7/9
2024-10-22 17 -
DNAPurificationfromAgaroseGels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide
2024-10-22 11 -
OligonucleotidePurification
Purpose: Purification of oligonucleotides (which have already been purified by r
2024-10-22 13 -
Plasmid Quickpreps
1.grow up single colony in 1.5 ml LB/antibiotic ovn @37℃ .2.pour into tube, spin
2024-10-22 12 -
BUCCAL CELL DNA PREPS
Important : Extract the DNA within one week of receiving samples. Samples that h
2024-10-22 12
