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Quick DNA Plasmid Prep
This protocol gives very clean plasmid preps for restriction digests and cloning
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DNAPurificationandPrecipitation
1.Prepare or obtain Buffered phenol,pH 8.0.Add 2 crystals of 8-Hydroxyquinoline
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MITOCHONDRIALDNAISOLATION
1.Grind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g t
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Dialysis (using dialysis tubing)
Molecularpourous membrane tubing is used for desalting, protein and DNA isolatio
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Generation of uni-directionalnesteddeletion sin plasmid DNA
1) Digest DNA with two restriction enzymes such that one end is ExoIII susceptib
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ExtractionfromCheekCellsDNA
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of an
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PurificationofPCRfragmentsforcloning
After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder
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De-phosphorylation of DNA
The preferred method of de-phosphorylation uses the buffer system described by P
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Recoveryof DNA from LMP(Low Melting Point)AgaroseGel
Recovery of DNA from LMP (Low Melting Point) Agarose Gel1. Separate DNA fragment
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Gene-Kleen protocol
Purification of DNA from agarose gels is an essential method involved in the sub
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IsolationofDNAfromMouseTai
Isolation of DNA from Mouse Tail Biopsies1. Obtain tail biopsies from 2 to 3 wee
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PROTOCOL TO EXTRACT DNA FROM PA
PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS1.Cut 10-20X10μm sections of form
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CTABTECHNIQUE Method Schedule Protocol(JPB)FOR DNA
CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTR
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BUCCAL CELL DNA PREPS
Important : Extract the DNA within one week of receiving samples. Samples that h
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Isolation of DNA from Acrylamide GELS
1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is
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