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ProtocolforIsolationofGenomicDNAfromMammalianCells
Protocol for Isolation of Genomic DNA from Mammalian Cells1.Trypsinize, harvest
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DNAFRAGMENTPURIFICATIONW/GLASSWOOL
Glass wool method:Run TAE agarose gel and cut the appropriate band out with a cl
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SilicaClean-upofDNA
Materials:Silica Suspension:add 2 g of silica to 15 ml of H2 Owash 3x by centifu
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DNAPurificationbyphenolextractionandethanolprecipitation
Equipment and reagents1. Phenol2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM E
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Isolating DNA Fragments
Materials:• 0.8 % agarose gel in 1x TAE• Digested DNA• Glass Milk
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Target enrich mentte chnologies for ABISOLiD
Q:my group is involved in a national project to develop a genomic platform for c
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Ribonuclease Protection Assay
1. Add appropriate volumes of RNA (<12 ul) plus probe (< 3 ul). Try 5 and
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Self-circularization of Linear DNA
一、In a microcentrifuge tube prepare a solution of linear DNA (25-50ng) in deioni
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Electro-elutionofnucleicacidsfromagaroseandpolyacrylamidegelslides
PrincipleThe nucleic acids contained within the gel slice are electrophoresed ou
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DNAISOLATIONFROMPRIMARYTUMORSVIACRYOSECTIONS
- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sec
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Improved Alcohol Precipitation of DNA
ECKDescriptionThis method was suggested in a BRL "Focus" article sever
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DNA Fragment Purification from Agarose or Acrylamide
DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to
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Preparation of GA Marker
Author: Long-Cheng LiSource: Protocol OnlineAbstract: Simplified method for prep
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RecoveringDNAfromagarosegels
IntroductionMethods and reagents is a unique monthly column that highlights curr
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Boilinglysismini-prep
1、Grow an overnight culture from a single colony in 2 mls LB antibiotic.2、Transf
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