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TACloning
TA CloningI. Initial mixture5 μl dd H2O1 μl PCR product1 μl ligation bu
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NH4AcandEtOHprecipitationofDNA
Add NH4Ac (10 M stock or solid) to the sample for a final concentration of 2.5 M
2024-10-21 10 -
ElectroelutionofDNAfromAgarose
Electroelution of agarose fragmentsElectroelution buffer1 M Tris, pH 7.5 12.0 ml
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Phlebotomus Sand flies的DNA的提取
1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50
2024-10-21 19 -
BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
1、A smear (rather than a single colony) of BAC colonies are picked and transferr
2024-10-21 15 -
DNA Extraction Methods
Phenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies i
2024-10-21 9 -
How to isolatesingle-stranded DNA frommammalian-Molec
Does anybody know how to isolate single-stranded DNA from mammalian cells?Thanks
2024-10-21 11 -
Spin Column chromatography
Used to removed unincorporated nucleotides from labelling reactions.Prepare Seph
2024-10-21 12 -
DNA Fragment Purification
TE solution10 mM Tris (pH to 7.5)1 mM EDTA (pH to 8.0 to dissolve)2.Frozen agaro
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Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydro
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FINGERPRINTING PROTOCOL(AGAROSEGEL)
FINGERPRINTING PROTOCOL (AGAROSE GEL) *Restriction digests consist of:15.75 ml d
2024-10-21 17 -
Phenol extraction of DNA samples
(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The Univer
2024-10-21 11 -
Ethanol Precipitation of DNA
Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasur
2024-10-21 10 -
DNA extraction from Mutation Detecti
Source: Contributed by Mohammad Reza Abbaszadegan et al. Abstract: Single strand
2024-10-21 19 -
Alternate Method for Purifying DNA
1.Excise band of interest from a TAE gel; estimate volume by weighing the gel sl
2024-10-21 12
