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  • CAT ASSAY[TLC METHOD]

    CAT ASSAY[TLC METHOD]

    CAT ASSAY[TLC METHOD]1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flask

    2024-10-21 11
  • Isolation of DNA from Mouse Tai

    Isolation of DNA from Mouse Tai

    Isolation of DNA from Mouse Tail Biopsies1. Obtain tail biopsies from 2 to 3 wee

    2024-10-21 13
  • DNA Fragment Purification

    DNA Fragment Purification

    TE solution10 mM Tris (pH to 7.5)1 mM EDTA (pH to 8.0 to dissolve)2.Frozen agaro

    2024-10-21 11
  • 如何来完成mRNA的纯化?

    如何来完成mRNA的纯化?

    mRNA的分离方法较多,其中以寡聚(dT)-纤维素柱层析法最为有效,已成为常规方法。此法利用mRNA 3’末端含有Poly(A+)的特点,在RNA流经寡聚(dT

    2024-10-21 12
  • Practical Tips for Optimal Northern Analysis

    Practical Tips for Optimal Northern Analysis

    Northern assays require RNA (total- or poly(A)- selected) to be resolved on a ag

    2024-10-21 12
  • CTAB Procedure

    CTAB Procedure

    In the hood:96 well dish with bacteriatitertechmicrotubesglass pipetteRemove col

    2024-10-21 10
  • Genomic (Plant) DNA: Restriction Digestion for Sou

    Genomic (Plant) DNA: Restriction Digestion for Sou

    Background on the art of getting beautiful Southerns:There are several things to

    2024-10-21 11
  • Tail Preps: DNA Isolation From

    Tail Preps: DNA Isolation From

    NOTE: THIS IS FINE FOR SOUTHERNS, BUT NOT FOR SCREENING BY PCR.(from Ruixia, 7/9

    2024-10-21 16
  • Filterbindingassay简介、原理和方法

    Filterbindingassay简介、原理和方法

    Filterbindingassay简介、原理和方法关键词: Filterbindingassay 原理 方法来源: 互联网简介优点:操作简单方便缺陷:不能像E

    2024-10-21 13
  • ES CELL DNA EXTRACTION: TUBE ME

    ES CELL DNA EXTRACTION: TUBE ME

    Protocol for extracting DNA from ES Cells, starting from the 96-well plate but p

    2024-10-21 18
  • DNA From Whole Blood for PCR

    DNA From Whole Blood for PCR

    1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapil

    2024-10-21 19
  • Plant Total DNA Isolation

    Plant Total DNA Isolation

    OverviewProcedure1. Preheat the CTAB Isolation Buffer at 60℃.2. Grind 2 g of fre

    2024-10-21 13
  • Southern Blot Protocol

    Southern Blot Protocol

    Day 11. Digest DNA for 6 hours (or overnight)BSA 10 mg/ml 0.5 22.65 ul of 10 ug

    2024-10-21 15
  • Largescale nuclear extract preparation

    Largescale nuclear extract preparation

    Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Kari

    2024-10-21 17
  • UV Quantitation of DNA

    UV Quantitation of DNA

    DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus

    2024-10-21 19