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MINI PREP BY BOILING
Pellet 1.5 ml of an overnight culture at 12,000 rpm in Eppendorf centrifuge at R
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Phenol/Chloroform Precipitation of DNA
1. Add an equal volume (equal to sample volume) of P/C to sample.2. Mix (shake,
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核酸抽提mRNA的分离技巧
mRNA的分离 与rRNA和tRNA不同的是，哺乳动物细胞的绝大部分mRNA在其3'端均有一poly(A)尾，因此可以用oligo(dT)－纤维素亲和层
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DNA Purification and Precipitation
1.Prepare or obtain Buffered phenol,pH 8.0.Add 2 crystals of 8-Hydroxyquinoline
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DNAExtractionProtocolsUsingSilica
Below we present the protocols we have used to isolate DNA from various tissues
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CLONING FROM LOW MELT AGAROSE
competent cells (DH5alpha at competency of 108 cfu/µl of pUC18). The transformat
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Isolation of DNA from Agarose Gels(Paper Slurry Method)
Isolation of DNA from Agarose Gels (Paper Slurry Method)This procedure isolates
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DNA Fragment Purification from Agaro
DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to
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DNA Extraction from Archival Formali
DNA Extraction from Archival Formalin-fixed, Paraffin-embed allowfullscreen='
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Rapid elution of DNA from agarose gels
Rapid elution of DNA from agarose gelsJames Movius, Hahn LabLast modified Sun, N
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Rapid elution of DNA from agaro
Rapid elution of DNA from agarose gelsJames Movius, Hahn LabLast modified Sun, N
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Yeast boiling DNA miniprep
Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992)Ha
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Aninexpen sivealter native to glass milk for DNA purificatio
Preparation of Silica1. Suspend 5 g of silica (Sigma, S-5631) in 50 ml of PBS.2.
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Purification of DNA from lambda phage
This is a modification of the procedure in Short Protocols (1-41,1-45)1.Prepare
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Oligonucleotide Purification
Purpose: Purification of oligonucleotides (which have already been purified by r
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