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WB 或 IP 样品制备的技术指导
在 WB 和 IP 实验中，破碎细胞或组织与选择和配制裂解液一样重要。比起一些更软的组织（如脑组织），在准备 WB 或 IP 的样品时，紧密的纤维组织（如肌肉组
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Western Blot转膜
在电流的作用下，使蛋白质从胶转移至固相载体（膜）上。膜的选择：印迹中常用的固相材料有NC膜、DBM、DDT、尼龙膜、PVDF等。我们选用PVDF（聚偏二氟乙烯）
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如何监控转膜的效率呢？
在早期，鄙人亦依据分子克隆的介绍，采用立春红染膜。只是后来发现此操作不仅增加额外的操作时间，而且不能说明任何问题，于是抛弃之。首先，立春红（也包括考染胶）的灵敏
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利用快速蛋白质层析系统（FPLC）分析啤酒中含氮化合物的组成
摘要：用蛋白质快速层析系统（FPLC）的几种层析技术对啤酒中的含氮化合物组成进行了研究。利用Suprose6/Superose12体积排阻柱对啤酒经透析得到的蛋
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Cyanogen Bromide digestion of protein
James Movius,Hahn Lab1.Proteins are TCA precipitated and washed with acetone,the
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Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase (TdT) is an enzyme that catalyzes the repe
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Glycolipid Binding Assay
ProcedureA: Preparation of the cell lysate1.Rinse a 60 mm culture dish of conflu
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Northern Blot
Northern Blot I. Electrophoresisclean gel box with NaOH and/or SDS, 2 hours to o
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James Hardwick's angiotensin assay protocol
This specific procedure was developed to assay the activity of the Lck kinase ex
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LABELING GIZZARD MYOSIN WITH IATR (MHC AND 17KD MLC)
Day 1-2Materials1.0.5 M KCl,10 mM HEPES,pH 7.5,4℃,250 ml.2.0.5 M KCl,50 mM HEPES
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Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluores
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Dried droplet
The discovery of this method allowed the application of laser desorption to prot
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DNA-Methyltransferase Assay
This protocol was written by Jean-Pierre Issa, based on Adams et al.* Kam-Wing J
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Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
Materials1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.St
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Western Blot of TBP from TBP-GST bacteria
Culture bacteria1. Grow glycerol stock A13 (TBP-GST in BL21) in 10ml LB and 10μl
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