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Western Blotting - Antibodies
1. Load gel in apparatus as described by manufacturer.2. Transfer proteins accor
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Gel Mobility Shift Analysis
Material/reagents:• 2X binding buffer: 20 mM Tris pH 7.5 100 mM NaCl
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Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml
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Troubleshooting Tips for Western Immunoblotting
The following tips can be used to overcome the most common problems encountered
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小鼠脑微血管内皮细胞(bEnd.3)
小鼠脑微血管内皮细胞(bEnd.3)小鼠脑微血管内皮细胞(bEnd.3)介绍：小鼠脑微血管内皮细胞(bEnd.3)转染了表达多瘤病毒中T 抗原的NTKmT 逆转
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Quantitation of Protein:Folin - phenol Reagent Method (Lowry Method)
【 Purpose 】 1.Study the principle and method of Lowry method to quantitate prote
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小鼠脑神经胶质母细胞瘤细胞(G422)
小鼠脑神经胶质母细胞瘤细胞(G422)小鼠脑神经胶质母细胞瘤细胞(G422)介绍：1964 年建株；甲基胆蒽植入Km 小鼠脑内诱发星形细胞瘤，传至第120 代后
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Western Blotting with Horseradish Pe
SAMPLE PREPARATIONFor Protein Concentration Determination of Cell Culture1.Decan
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小鼠小胶质细胞(BV2)
小鼠小胶质细胞(BV2)小鼠小胶质细胞(BV2)介绍：小鼠小胶质细胞(BV2)来源于德国DSMZ（German collection of microorgan
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小鼠颅顶前骨细胞亚克隆14(MC3T3E1 Subclone14)
小鼠颅顶前骨细胞亚克隆14(MC3T3E1 Subclone14)小鼠颅顶前骨细胞亚克隆14(MC3T3E1 Subclone14)介绍：从克隆的但是表型各异的
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小鼠胚胎成纤维细胞(3T3-L1)
小鼠胚胎成纤维细胞(3T3-L1)小鼠胚胎成纤维细胞(3T3-L1)介绍：L1 是通过克隆分离得到的3T3 (swiss 小白鼠)的连续亚株。当细胞从快速分裂到
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Troubleshooting Guide: Western Blot
Use alternate buffer system (such as CAPS buffer pH 10.5) with a higher pH.Assem
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小鼠胚胎成纤维细胞(BALB/3T3 clone A31)
小鼠胚胎成纤维细胞(BALB/3T3 clone A31)小鼠胚胎成纤维细胞(BALB/3T3 clone A31)介绍：小鼠胚胎成纤维细胞(BALB/3T3
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Western Blotting with Biotinylated Antibodies
SAMPLE PREPARATIONFor Protein Concentration Determination of Cell Culture1.Decan
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小鼠胚胎成纤维细胞（MEF）
小鼠胚胎成纤维细胞（MEF）小鼠胚胎成纤维细胞（MEF）介绍：取孕9天的615小鼠胚胎，去除脑、心脏等培养建立。该细胞可用作饲养层细胞，支持胚胎干细胞ES的生长
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