-
Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydro
2024-10-17 14 -
DNA Purification by phenol extraction and ethanol precipitation
Equipment and reagents1. Phenol2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM
2024-10-17 15 -
Mini DNA extraction from wheat leaves for PCR and Southern B
Procedure for 100 ~ 300 mg of tissue.1. Collect fresh tissue, coleoptiles or sma
2024-10-17 16 -
UV Quantitation of DNA
DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus
2024-10-17 19 -
NH4Ac and EtOH precipitation
NH4Ac and EtOH precipitation of DNAAdd NH4Ac (10M stock or solid) to the sample
2024-10-17 16 -
Mag-Bind Soil DNA Kit Protoco
1. Weigh 500 mg of glass beads in a 2 ml centrifuge tube, add 0.2-0.5 g soil sam
2024-10-17 14 -
Boiling lysis mini-prep
1、Grow an overnight culture from a single colony in 2 mls LB + antibiotic.2、Tran
2024-10-17 15 -
Concentration of DNA by ethanol precipitation
(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The Univer
2024-10-17 17 -
Purification of PCR fragments for cloning
After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder
2024-10-17 19 -
DNA Plasmid Miniprep Protocol
1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l ampicill
2024-10-17 15 -
DNA extraction from blister rust aeciospores and urediniospores using dry grindi
Most recent version of protocol: 2/14/96Dr. Paul J. Zambino, Research Plant Mole
2024-10-17 15 -
Diatomaceous Earth-based Midi-prep
Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice
2024-10-17 12 -
Genome Walking Kit 使用注意
用于Genome Walking Kit的模板总DNA 一般要满足以下几点：1、DNA 的完整性，电泳为单一条带。2、避免杂质，OD260/280=1.8~2.
2024-10-17 12 -
Increasing Sensitivity in Northern Analysis with RNA Probes
Note: The pointers in this technical bulletin presuppose the use of standard 50%
2024-10-17 11 -
Histochemical detection重组质体的检定
操作原理与colony hybridization 相同，但是将滤膜移置于含有IPTG 及5-bromo-4-chloro-3-indolyl β-D-gluc
2024-10-17 19
