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RNA purification --- Trizol
1. Snap freeze ~107 cells or ~100 mg of tissue in liquid nitrogen or dry ice/eth
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RNA提取--RNA ISOLATION
DEP treatment solutions. Add 0.3 ml lysing buffer (0.1 M Hepes, 0.2 M EDTA, 10%
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按大小分离RNA
乙二醛化RNA琼脂糖凝胶电泳和含有甲醛的琼脂糖凝胶电泳均可按大小分离RNA。RNA样品经甲酰胺处理以及经含有甲醛的凝胶电泳分离可能会发生变性,但因为变性RNA比
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啤酒酵母(S. cerevisiae)mRNA的提取
Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Col
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oligo(dT)-纤维素层析法提取poly(A)+RNA
与rRNA,5SRNA,5.8SRNA和tRNA相比,大多数真核生物的mRNA在其3’端带有poly(A)尾巴,因此mRNA能用oligo(dT)-纤维素亲和层
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肝中RNA的分离与鉴定
原理细胞内的核酸多以核蛋白的形式存在,其中脱氧核糖核蛋白主要存在于细胞核中,核糖核蛋白主要存在于细胞质中。这两类核蛋白在0.14mol/L氯化钠溶液中的溶解度相
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科研中的“新星”技术——MicroRNA荧光定量PCR
Micro RNA简介微小RNA(microRNA,简称miRNA)是生物体内源长度约为20-23个核苷酸的非编码单链小分子RNA,通过与靶mRNA的互补配对而
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成熟针叶林针叶和针叶树韧皮组织总RNA提取
Many different methods have been tried in an effort to extract quality RNA from
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Isolation of total RNA using CsCl/EtBr gradients--密度梯度法提取总RNA
RNA for S1 or PE analysis must be free of chromosomal DNA. This can be accomplis
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TRIzol method for RNA isolation
TRIzol method for RNA isolation-To tissue or cells, add 200- 1 ml TRIzol (GIBCO
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提取棉花RNA--RNA EXTRACTION METHOD FOR COTTON
Based on: Wan, C.-Y. and Wilkins, T.A. 1994. Anal. Bioch. 223:7-12.1.Collect you
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从土壤中轻松获取高质量RNA
众所周知,从土壤中提取DNA已非易事,而想从中再提取RNA可说是难上加难。DNA不容易提取是因为本身土壤中富集的材料就少,提取过程相对复杂,造成操作损失;而RN
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How to isolate mRNA(分离mRNA)
Background: mRNAs (messenger RNAs) comprise only a small percentage of all RNA s
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CONCERT细胞质RNA纯化试剂
CONCERT细胞质RNA 纯化试剂是一种新颖的单相酚溶液,是专门设计用来从新鲜或冷冻的培养的动物细胞 或组织中快速、简单地纯化高质量的细胞质RNA 。应用利用
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变性胶体电泳与北方杂合分析
mRNA为单股,一般会卷绕成特定的三级结构,并与某些蛋白质结合,而以messenger ribonucleoprotein particles (mRNPs)
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