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PROTOCOL TO EXTRACT DNA FROM PA
PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS1.Cut 10-20X10μm sections of formali
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MCA
2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polymeras
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S1 Nuclease Protection Assay
End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci
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DNA Plasmid Prep.
This is a scaled up version of the Baron protocol which has been modified to ach
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PEG Preparation of Plasmid DNA
PEG Preparation of PlasmidPlasmid isolated by this procedure can be used routine
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DNA Purification from Gels
1. Remove gel slice contain DNA fragment and place in 10 volumes of:300 mM NaOAc
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Alkaline Southern Blotting Procedure
Author: Suzanne GerttulaDate: March 14,1994Digest between 1 and 10 ug Genomic DN
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Electro-elution of nucleic acids from agarose and polyacrylamide gel slides
PrincipleThe nucleic acids contained within the gel slice are electrophoresed ou
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ES CELL DNA EXTRACTION: TUBE ME
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but p
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Genomic DNA Quickprep for PCR
macerate tissue in Eppendorf tube without butter at RTadd 400 m l extraction buf
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RLGS protocol
The principle and procedures of RLGS method was first described by Hatada et al.
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Metaphase chromosome preparation
Materials:RPMI 1640 mediumfetal calf serum (FCS), 20%Colcemid (e.g. Boehringer M
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DNA extraction from Mutation Detecti
Source: Contributed by Mohammad Reza Abbaszadegan et al. Abstract: Single strand
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BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
1、A smear (rather than a single colony) of BAC colonies are picked and transferr
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Isolating DNA Fragments
Materials:• 0.8 % agarose gel in 1x TAE• Digested DNA• Glass Milk• NaI solution•
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