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Genomic DNA Extraction for Mapping
1.grow plants in trays of 96 and leave two spots open (for the PCR controls)2.ha
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Diatomaceous Earth-based Midi-prep
Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice
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DNA Preparation from Fresh/Frozen Tissue
ReagentsChloroform EDTA,0.5 M Ethanol, absolute Isoamyl alcohol
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DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
This is a four day procedure so it's best to start on Monday or Tuesday.CASE
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DNA Purification-Modified CTAB Procedure
This protocol originated in Murray and Thompson (1980) and then was modified by
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Analys of Genomic DNA by Southern Hybridization
Localization of particular sequences within genomic DNA is usually accomplished
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PREPARATION OF PLASMID DNA FOR SEQUENCING
The following protocol is based on our modifications of R. Kraft, J. Tardiff, K.
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Plasmid Mini-prep Protocol
1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on hi
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DNA Extraction Methods
Phenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies
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碱裂解法制备大肠杆菌质粒（Alkaline Lysis Plasmid Preparation）
This protocol yields plasmid DNA that is suitable for restriction digests and cl
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DNA的琼脂糖纯化(John Roth, Division of Biological Sciences,U
1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.
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从琼脂糖和丙烯酰胺中纯化DNA
For fragments from 200 bp to 10 kb the agarose purification is ideal. For smalle
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Extraction of DNA from Schizophyllum commune （裂褶菌DNA提取）
1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plat
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Oligonucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
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DNA isolation
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION
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