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Procedure to purify protein from cells
This procedure is used to break cells that contain a protein to be purified.This
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Preparation of Dialysis tubing
1)Boil the tubing on a sitr plate (preferably in the hood)in a 4L volume of 2% (
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Myosin Light Chain Preparation
To be done the day before:1.Put meat grinder and accessories in the cold room.2.
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Purification of TFIIIC
SummaryWCE Extracts are prepared from strain SHY282 (TFC4-Flag-EE (HpaI)).TFIIIC
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Preparation of Affinity Column制备亲和层析柱【UCSF】
1.Add 222.2μl 1M MOPS,pH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final
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Crystallization Trials
MaterialsMilipore filter type HA 0.45 micronCulture plates (Linbro model 76-033-
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Preparation of Cell Lysate
1. Wash adherent cells twice in the dish or flask with ice-cold PBS and drain of
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Purification of recombinant sBRF M166L
J. Movius, K, Coachman, and S. Hahn (Hahn Lab)Induction of BRF in bacteria and p
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Preparation of Cell Lysate
1.Wash adherent cells twice in the dish or flask with ice-cold PBS and drain off
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Purification of dnEBNA-1/Soft from E. coli BL21 LysS
Inoculate 2ml of 5ml o/n culture of either p3133 (empty vector pET11a)or p3134 (
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Rapid Yeast Protein Prep for SDS PAGE and Western
Sample Buffer:10 ml0.06M Tris-HCl, pH 6.80.6 ml 1M Tris 6.810% (v/v) glycerol2 m
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Large scale nuclear extract preparat
Large scale nuclear extract preparat关键词： nuclear extract preparat来源： 互联网Hattoti&
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Purifying Protein from Inclusion Bodies
Buffer ABuffer B50 mM tris-HCl, pH 8.020 mM Na2 HPO4 , pH 7.25 mM EDTA20 mM NaCl
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Preparation of protein extracts for western blot
1.Grow cells to mid-log (OD600 less or equal to 1.0).2.Harvest about 5 OD's
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Urea Lysis Protocol
9M Urea,2.5mM EDTA,2.5mM EGTA,1% DTE,4% CHAPSmake 10ml and aliquot 10x1ml,freeze
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