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详细BAC DNA Isolation
A. Culture Growth:1.For BAC isolation for shotgun library construction:Pick a sm
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Simplified DNA Extraction from Cell or Tissue
PurposeDNA extraction without phenol extraction and centrifugation.Procedure1. L
2024-10-17 9 -
DNA Recovery With Low Melt Agarose
Recovery of DNA from Low Melting Point Agarose Gels1.Run digestion products on 0
2024-10-17 9 -
Preparation of BAC DNA with by Alkaline Prep Method
CLONE ISOLATION PREP - PROTOCOLBefore you begin isolating DNA from the cultures:
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Yeast Nuclear Extract (Small Scale)
Hahn Lab (adapted from J.Leatherwood,Ptashne lab)last modified Mon,Aug 27,2001Ce
2024-10-17 14 -
RNAi Glossary
Argonaute - A family of proteins containing multiple domains and involved in RNA
2024-10-17 13 -
MinElute DNA纯化
做分子生物学实验,核酸纯化、酶切、克隆算是最基本的步骤了。因为要做定向克隆,通常要作双酶切。为了省时省事儿,我们常常想方设法把两个酶放在一起切。可是事情并非总
2024-10-17 16 -
植物基因组的快速提取
Use from 0.01 - 0.1 gram plant material.Grind the plant material with liq. N2 in
2024-10-17 10 -
琼脂糖凝胶回收DNA――纸浆法
This procedure isolates DNA from agarose gels by filtration through a filter-pap
2024-10-17 12 -
MinElute DNA纯化技术
作分子生物学实验,核酸纯化、酶切、克隆算是最基本的步骤了。因为要做定向克隆,通常要作双酶切。为了省时省事儿,我们常常想方设法把两个酶放在一起切。可是事情并非总那
2024-10-17 13 -
DNA extraction from Mutation Detection Enhancement (MDE) Gel
Abstract: Single strand conformational polymorphism (SSCP)is the most widely use
2024-10-17 11 -
E-Z 96 Plant DNA Protocol-Vacuum Manifold Processing
Note: The following protocol is based on using OBI’s vacuum manifold (Product No
2024-10-17 16 -
病毒DNA和RNA的简易纯化技术
EZ1 病毒迷你试剂盒( EZ1 Virus Mini Kit )是一种能够用于生命科学研究中的病毒 DNA 和 RNA 提纯的新型试剂盒。这种试剂盒提供了一种
2024-10-17 16 -
Ethanol Precipitation of DNA
This procedure allows the concentration of DNA samples from dilute solution and
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DNA Isolation from Tails
Jacks Lab,Center for cancer research,MIT1.Each tail should be in a clean eppendo
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