-
植物基因组DNA提取
【实验目的】 掌握植物总DNA的抽提方法和基本原理。学习根据不同的植物和实验要求设计和改良植物总DNA抽提方法。 【实验原理】 通常采用机械研磨的方法破碎植物的
2024-10-17 14 -
A quick method to isolate plant
Use from 0.01 - 0.1 gram plant material.Grind the plant material with liq.N2 in
2024-10-17 9 -
Extraction of Genomic DNA from Mouse Tail
1、Put a 0.5-1.5 CM MOUSE TAIL SNIP in a 1.5 ml eppindorf tube and store frozen u
2024-10-17 44 -
Plasmid DNA Isolation, Alkaline Lysis（碱裂解法分离质粒DNA）
Reagents:Soln 1: 50 mM glucose/10 mM EDTA/25 mM Tris pH 8. Autoclave before use.
2024-10-17 17 -
Isolation of Retroelement from Plant Genomic DNA (PCR法从植物寻找逆转录元件)
Retroelements and their derivatives are a ubiquitous and abundant component of p
2024-10-17 17 -
Use of Internal and External Standards or Reference RNAs for
IntroductionNorthern blotting, nuclease protection assays and RT-PCR are frequen
2024-10-17 14 -
Genomic DNA Quickprep for PCR
macerate tissue in Eppendorf tube without butter at RTadd 400 m l extraction buf
2024-10-17 16 -
Phenol/chloroform Extraction of DNA
Materials:phenol:chloroform (1:1) chloroformAdd an equal volume of buffer-satura
2024-10-17 15 -
煮沸裂解法小量制备质粒DNA
经Triton X-100、溶菌酶和加热处理可从小量（1～2ml）细菌培养物中分离质粒DNA所获得的质粒则可以用电泳或限制性内功核酸酶消化的方法鉴定；经PEG处
2024-10-17 15 -
RNase Protection Assay (Bowtell Lab)
1. Cut 10ug of plasmid DNA where you want the transcript to end.2. Add an equal
2024-10-17 14 -
DNA Preparation from Adherent Cells
Section of Cancer Genomics,Genetics Branch,NCINational Institutes of HealthReage
2024-10-17 16 -
CAT ASSAY[TLC METHOD]
CAT ASSAY[TLC METHOD]1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flask
2024-10-17 10 -
冷冻组织DNA的提取
Tissue collection, storage, microdissection, sectioning: See separate protocol.
2024-10-16 20 -
DNA Extraction
This is a modification of a salting out procedure as described by Miller et al.,
2024-10-16 16 -
RAPD
一、 材料 不同来源的DNA(50ng/ul)。二、设备 PCR仪，PCR管或硅化的0.5ml eppendorf管，电泳装置。三、试剂 1、随机引
2024-10-16 16
