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Thermal Inactivation
A simple, reversible way to a stop restriction reaction is by adding EDTA, which
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Partial Endonuclease Digestion
Prepare a 100 µl reaction mixture containing the DNA of interest in the appropri
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EXO/S1 DELETION SERIES
1. Cut DNA with 5'- and 3'- overhangs, gel check, phenol-Sevag extract
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NEB识别序列
下表中列出了NEB提供的所有限制性内切酶的特异性识别序列，同时列出的还有回文序列的每条互补链。比如CCTC(7/6) 和(6/7)GAGG均显示为MnlI识别序
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Restriction Digestion of DNA
Restriction enzymes are expensive ($40 to $200 a vial): keep the enzymes at -20℃
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UV Shadowing
UV shadowing is a technique for visualizing nucleic acids separated on acrylamid
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FOOTPRINTING WITH DNASE1
1)probe: same as used for gelshift), isolated by isotacelectrophoresis w/out EtO
2024-10-23 21 -
DNA rapid CTAB
A Rapid CTAB DNA Isolation TechniqueUseful for RAPD Fingerprinting and Other PCR
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连接互相匹配的粘性末端，产生新的酶切位点
通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可
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创造新的酶切位点
将互相匹配的DNA末端连接起来可以产生新的酶切位点。这些DNA末端可以通过以下方法获得： 1. 补平5′突出的粘性末端产生平末端 2. 双酶切产生的平末端 3.
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Restriction digestion
Restriction enzyme digestions are performed by incubating double-stranded DNA mo
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Partial Endonuclease Digestion
Prepare a 100 µl reaction mixture containing the DNA of interest in the appropri
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Restriction Enzyme Digestion of DNA
Materials:10X restriction enzyme buffer (see manufacturer's recommendation)D
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DNA 的定量与连接反应
药品试剂：纯化的gusA 片段及pQE31 BamHI/HindIII 片段1% 6-well agarose gelDNA 标准分子量 （λMr, 0.5 μ
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Restriction Enzyme Buffer
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are
2024-10-23 16
