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PlasmidMini-prepProtocol
1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on hi
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Phenol extraction of DNA samples
(adapted from Bruce A.Roe,Department of Chemistry and Biochemistry,The Universit
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BACDNAIsolationwithAutoGen740
1、The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB
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PlasmidDNAminipreps
There are many methods for DNA preparation without using kits.Below are a few mi
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Preparation of Mouse Tail DNA for Dot Blots or PCR
These procedures were originally devised in Richard Palmiter's lab for use w
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Streamlined DNA Extraction Protocol
This method is derived from a procedure developed by Toby Bradshaw and the Popla
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An inexpensive alternative to glassmilk for DNA purification
Preparation of Silica1. Suspend 5 g of silica (Sigma, S-5631) in 50 ml of PBS.2.
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Strategy for accurate sequencing through hairpin structures
Verifying the sequence of an shRNA hairpin is essentialsince mismatch of even on
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Silica Clean-up of DNA
Silica Suspension:add 2 g of silica to 15 ml of H2Owash 3x by centifugation at 2
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CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA
CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTR
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Southern blotting实验
PURPOSE: TolocateaparticularsequenceofDNAwithinacomplexmixture(locateonegenewith
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DNA Extraction from Frozen Tissue Sections
Tissue collection, storage, microdissection, sectionin g: See separate protocol.
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Chromosomal DNA Extraction from Gram-positive Bacteria
This procedure was originally developed for Listeria monocytogenes but has worke
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Protocol for Isolation of Genomic DNA from Mammalian Cells
Protocol for Isolation of Genomic DNA from Mammalian Cells1.Trypsinize, harvest
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Dialysis (using dialysis tubing)
Molecularpourous membrane tubing is used for desalting, protein and DNA isolatio
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