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乙醇沉淀DNA实验操作方法
1. 加入 1/10 体积的乙酸钠（3 mol/L ，PH=5.2）于 DNA 溶液中充分混匀，使其 最终浓度为 0.3 mol/L； 2. 加入 2 倍体积
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DNA抽提指南(TRIZOL)
按照RNA 分离操作方案在完全移去水样层后，匀浆中的DNA 存在于中间层和苯酚层中也可以被分离出来。在沉淀和多次洗脱后，DNA 溶解在8 mM NaOH中。用
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Chemical Sequencing of DNA
This is a rapid method for chemical DNA sequencing which is commonly used as lad
2024-10-11 11 -
PREPARATION OF SEQUENCING GELS
MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or
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DNA SEQUENCING REACTIONS
DNA priming reactionx ul DNA 2 ul Reaction buffer, minus DTT 1 ul prim
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NO "WEDGE" Sequencing Gels
I have been using an alternative to wedge gels that saves acrylamide, cuts gel d
2024-10-11 11 -
Chemical Sequencing of DNA
This is a rapid method for chemical DNA sequencing which is commonly used as lad
2024-10-11 11 -
DNA Sequencing Gels
Buffers and gel solutions Long Ranger: we started using this in early 1995. Grea
2024-10-11 9 -
SEQUENCING GELS
10X TBE, per liter Sequencing dye ------------------- ----------
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Plasmid Sequencing
This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Pr
2024-10-11 11 -
DNA Sequencing Gels
Buffers and gel solutions Long Ranger: we started using this in early 1995. Grea
2024-10-11 12 -
DNA内电子传递
ＤＮＡ的双螺旋结构中碱基对在整个大分子链形成一个大的pai键 这个结构在导电高分子中很常见，这个键就是导电高分子导电的核心关键，而ＤＮＡ中的这个大pai键的作用
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DNA SEQUENCING (SANGER)
1. For double-stranded DNA templates:a. Denature template: 10ml DNA (5-10g or al
2024-10-11 11 -
有效提高非模式生物蛋白质组鉴定的新策略 ——逐次修正基因组法
随着高通量测序技术的不断崛起，全基因组测序也逐步普及。越来越多的物种基因组予以公布。目前，主要有两种获得研究物种参考基因组的策略：de novo 基因组拼接和基
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DNA SEQUENCING(SANGER)
1. For double-stranded DNA templates:a. Denature template: 10ml DNA (5-10g or al
2024-10-11 14
