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Increasing Sensitivity in Northern Analysis with RNA Probes
Note: The pointers in this technical bulletin presuppose the use of standard 50%
2024-10-17 12 -
Histochemical detection重组质体的检定
操作原理与colony hybridization 相同，但是将滤膜移置于含有IPTG 及5-bromo-4-chloro-3-indolyl β-D-gluc
2024-10-17 20 -
PROTOCOL TO EXTRACT DNA FROM PA
PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS1.Cut 10-20X10μm sections of formali
2024-10-17 15 -
MCA
2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polymeras
2024-10-17 16 -
S1 Nuclease Protection Assay
End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci
2024-10-17 20 -
DNA Plasmid Prep.
This is a scaled up version of the Baron protocol which has been modified to ach
2024-10-17 16 -
PEG Preparation of Plasmid DNA
PEG Preparation of PlasmidPlasmid isolated by this procedure can be used routine
2024-10-17 17 -
DNA Purification from Gels
1. Remove gel slice contain DNA fragment and place in 10 volumes of:300 mM NaOAc
2024-10-17 10 -
Alkaline Southern Blotting Procedure
Author: Suzanne GerttulaDate: March 14,1994Digest between 1 and 10 ug Genomic DN
2024-10-17 12 -
Electro-elution of nucleic acids from agarose and polyacrylamide gel slides
PrincipleThe nucleic acids contained within the gel slice are electrophoresed ou
2024-10-17 14 -
ES CELL DNA EXTRACTION: TUBE ME
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but p
2024-10-17 17 -
Genomic DNA Quickprep for PCR
macerate tissue in Eppendorf tube without butter at RTadd 400 m l extraction buf
2024-10-17 15 -
RLGS protocol
The principle and procedures of RLGS method was first described by Hatada et al.
2024-10-17 17 -
Metaphase chromosome preparation
Materials:RPMI 1640 mediumfetal calf serum (FCS), 20%Colcemid (e.g. Boehringer M
2024-10-17 16 -
DNA extraction from Mutation Detecti
Source: Contributed by Mohammad Reza Abbaszadegan et al. Abstract: Single strand
2024-10-17 16
