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  • RT-PCR Analysis--详细的RT-PCR方法

    RT-PCR Analysis--详细的RT-PCR方法

    Solutions10X RT Buffer10X PCR Buffer100 mM Tris pH 9.0500 mM KCl1% Triton X-1002

    2024-11-19 26
  • RT-PCR: The Basics

    RT-PCR: The Basics

    RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive t

    2024-11-19 22
  • What is the highest temperature&nbsp

    What is the highest temperature&nbsp

    QuestionWhat is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSCRI

    2024-11-19 24
  • Real-Time or Kinetic PCR

    Real-Time or Kinetic PCR

    The DNA Facility houses the “real-time” or kinetic PCR instrument, the Applied B

    2024-11-19 24
  • PCR Amplification from Microbial Colonies

    PCR Amplification from Microbial Colonies

    RAPD PCR Colony MiniprepEdit the program or pick up a program (No 10) if the pro

    2024-11-19 23
  • Salt (KCl) concentration

    Salt (KCl) concentration

    For the successful PCR or multiplex PCR amplification of many loci (especiall

    2024-11-19 23
  • Polymerase Chain Reaction

    Polymerase Chain Reaction

    Materials:Supplies: Procedures: Results: 上一篇:PCR Amplification of DNA 下一篇:Stan

    2024-11-19 23
  • Calculating Concentrations for PCR

    Calculating Concentrations for PCR

    a) Primers :i) Oligonucleotide primers are generally supplied as "so many O

    2024-11-19 26
  • PURIFICATION OF PCR PRODUCTS WITH SEPHAD

    PURIFICATION OF PCR PRODUCTS WITH SEPHAD

    Place the sephadex measuring plate (MultiScreen ?Column Loader) on a clean piece

    2024-11-19 39
  • Primer amount in PCR

    Primer amount in PCR

    Absolute value of primer concentration in multiplex PCR . The amount of DNA pri

    2024-11-19 29
  • Preparation of G+A Marker

    Preparation of G+A Marker

    Preparation of G+A Marker   Author: Long-Cheng Li   Source: Protocol Online

    2024-11-19 28
  • RT-PCR --- one-step

    RT-PCR --- one-step

    This protocol uses C. therm. Polymerase One-Step RT-PCR system from Roche.1. Set

    2024-11-19 25
  • PCR with expand Polymerase

    PCR with expand Polymerase

    Expand High Fidelity PCR (Boehringer)Steve HahnLast modified 12/12/99Expand is a

    2024-11-19 26
  • Multiplexing primer pairs

    Multiplexing primer pairs

    Single locus PCR. First step in designing a multiplex PCR is choosing the primer

    2024-11-19 24
  • 幽门螺杆菌的基因诊断

    幽门螺杆菌的基因诊断

    幽门螺杆菌的基因诊断   早在19世纪未,就有注意到胃粘膜组织中存在一种螺旋形微生物,其后陆续有类似的报道,但均未引起人们的足够重视.1983年,澳大利亚学者W

    2024-11-19 28