-
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
1、A smear (rather than a single colony) of BAC colonies are picked and transferr
2024-10-17 19 -
Isolating DNA Fragments
Materials:• 0.8 % agarose gel in 1x TAE• Digested DNA• Glass Milk• NaI solution•
2024-10-17 15 -
Genomic DNA Extraction for Mapping
1.grow plants in trays of 96 and leave two spots open (for the PCR controls)2.ha
2024-10-17 11 -
Diatomaceous Earth-based Midi-prep
Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice
2024-10-17 16 -
DNA Preparation from Fresh/Frozen Tissue
ReagentsChloroform EDTA,0.5 M Ethanol, absolute Isoamyl alcohol
2024-10-17 11 -
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
This is a four day procedure so it's best to start on Monday or Tuesday.CASE
2024-10-17 14 -
DNA Purification-Modified CTAB Procedure
This protocol originated in Murray and Thompson (1980) and then was modified by
2024-10-17 12 -
Analys of Genomic DNA by Southern Hybridization
Localization of particular sequences within genomic DNA is usually accomplished
2024-10-17 11 -
PREPARATION OF PLASMID DNA FOR SEQUENCING
The following protocol is based on our modifications of R. Kraft, J. Tardiff, K.
2024-10-17 12 -
Plasmid Mini-prep Protocol
1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on hi
2024-10-17 20 -
DNA Extraction Methods
Phenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies
2024-10-17 16 -
碱裂解法制备大肠杆菌质粒（Alkaline Lysis Plasmid Preparation）
This protocol yields plasmid DNA that is suitable for restriction digests and cl
2024-10-17 20 -
DNA的琼脂糖纯化(John Roth, Division of Biological Sciences,U
1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.
2024-10-17 12 -
从琼脂糖和丙烯酰胺中纯化DNA
For fragments from 200 bp to 10 kb the agarose purification is ideal. For smalle
2024-10-17 9 -
Extraction of DNA from Schizophyllum commune （裂褶菌DNA提取）
1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plat
2024-10-17 14
