-
Oligonucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
2024-10-17 10 -
DNA isolation
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION
2024-10-17 14 -
Cetyltrimethylammoniumbromide(CTAB)Pla
Procedure1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid
2024-10-17 14 -
Quick DNA Plasmid Prep
This protocol gives very clean plasmid preps for restriction digests and cloning
2024-10-17 13 -
Single Strand DNA Prep. for Sequencing
This protocol works well from a 5 ml starting culture or a 1 ml starting culture
2024-10-17 10 -
DNA Extraction from Cheek Cells
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of an
2024-10-17 10 -
Concentration of DNA by ethanol precipitation
(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The Univer
2024-10-17 12 -
Plasmid DNA Isolation from Bacteria
Materials:TE buffer10% (w/v) sodium dodecyl sulfate (SDS)20 mg/ml proteinase Kp
2024-10-17 10 -
Extraction of genomic DNA from whole blood
Abstract: The protocol is simple and fairly rapid.It does not require the use of
2024-10-17 13 -
Mag-Bind RX Plasmid Isolation Protocol
一、实验试剂 Absolute (96%-100%) ethanol 二、实验设备 1. Centrifuge with swinging-bucket
2024-10-17 13 -
PEG Preparation of Plasmid DNA
PEG Preparation of PlasmidPlasmid isolated by this procedure can be used routine
2024-10-17 20 -
Membrane Transfer and Crosslinking for RNA
Analysis of mRNA expression in tissue or cell culture is often done by Northern
2024-10-17 16 -
Silica Clean-up of DNA
Materials:Silica Suspension:add 2 g of silica to 15 ml of H2Owash 3x by centifug
2024-10-17 19 -
Electroelution of DNA from Agarose
Electroelution of agarose fragmentsElectroelution buffer1 M Tris, pH 7.5 12.0 ml
2024-10-17 13 -
silica回收DNA片段
1、在长波紫外灯下切下含有目标DNA的琼脂糖凝胶， 用纸巾吸干凝胶表面的液体并切小。计算凝胶的重量。2、加入2-3倍体积的6M KI或NaI（避光4度保存）。3
2024-10-17 16
