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  • Blunt end cloning of PCR produc

    Blunt end cloning of PCR produc

    End repair: Add 5-10 units of T4 DNApol and incubate at 37C for 5 minutes. Make

    2024-11-19 24
  • Single Cell PCR

    Single Cell PCR

    Single Cell PCR(Protocol provided by Carolyn Troeger)Cell picking c Axiovert 100

    2024-11-19 20
  • PCR基本实验方法(五)

    PCR基本实验方法(五)

    PCR基本实验方法(五)关键词: PCR 基本实验方法来源: 互联网Cloning PCR ProductsT-A Cloning Strategy: Taq

    2024-11-19 21
  • Primary Amplification of Genomic DNA using DOP - PCR

    Primary Amplification of Genomic DNA using DOP - PCR

    Reagents Agarose, Ultrapure Gibco, BRL, Cat. no. 15510-027 10X Buffer, and Perki

    2024-11-19 16
  • Polymerase Chain Reaction (PCR) cont

    Polymerase Chain Reaction (PCR) cont

    Polymerase Chain Reaction (PCR) cont. Choice of Polymerases for PCROne of the i

    2024-11-19 28
  • RAPD PCR Colony Miniprep

    RAPD PCR Colony Miniprep

    Edit the program or pick up a program (No 10) if the program has been set. Rea

    2024-11-19 20
  • Detection of Alu by PCR

    Detection of Alu by PCR

    Detection of Alu by PCR A Human DNA Fingerprinting Lab Protocol 1994 Cold Spring

    2024-11-19 23
  • PCR技术在血友病A基因

    PCR技术在血友病A基因

    PCR技术在血友病A基因诊断中的应用   血友病A是常见的遗传性凝血障碍,它是由凝血因子Ⅷ的基因缺陷而致其功能异 常的致其发病率改为1/1000男性,女性患者极

    2024-11-19 26
  • Choosing/designing PCR primers

    Choosing/designing PCR primers

    In designing primers for PCR, the following steps/rules were tested and proven t

    2024-11-19 27
  • "BEST" PCR--从质粒上扩增DNA的PCR条件

    "BEST" PCR--从质粒上扩增DNA的PCR条件

    BEST" PCR conditions for amplifying DNA from plasmids25 ng linear template

    2024-11-19 18
  • THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR

    THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR

    TABLE OF CONTENTS 1. Abstract 2. Introductory statement 3. The key preparatory s

    2024-11-19 22
  • Protocol for Enhancing PCR of Very Difficult Regions

    Protocol for Enhancing PCR of Very Difficult Regions

    Protocol for Enhancing PCR of Very Difficult Regions Ziyun Yao, Shaoping Lin, Ho

    2024-11-19 30
  • Quantification of PCR using delta-delta Ct method.-Molec

    Quantification of PCR using delta-delta Ct method.-Molec

    One of the most used quantification methods in PCR is the "delta-delta Ct&q

    2024-11-19 20
  • is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea

    is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea

    The gene we are interested in looking at is not very abundant. At 100 ng/ul, the

    2024-11-19 24
  • SDS-PAGE--聚丙烯凝胶电泳

    SDS-PAGE--聚丙烯凝胶电泳

    DS-PAGE: gel electrophoresis of proteins TECHNIQUE is to set up gel plates befor

    2024-11-19 30