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TMCF Protocols: Southern Blotting
BUFFERS:1) Prehybridization/hybridization buffer1% SDS6X SSC10 % dextran sulfate
2024-10-09 13 -
southern blots-Molecular Biology
Hi can anyone answer thisI am having problems with my southern hybridization.I a
2024-10-09 17 -
RNA/Zeta Probe Dot Blotting protocol
RNA/Zeta Probe Dot Blotting protocol1)Make up RNA (up to 20μg)dissolved in steri
2024-10-09 17 -
Southern Blot
1. Run gel (0.8 -1.0% agarose is best). For yeast chromosomal Southerns, digest
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genomic DNA digest-Molecular Biology
I am trying to digest mouse genomic DNA for a southern blot. When digested with
2024-10-09 15 -
Radiolabeling of probes for electrophoretic mobility shift assays
Materials:g -32 P ATP (New England Nuclear/Perkin Elmer, CAT# NEG-035C)“Upper s
2024-10-09 14 -
Southern protocol-Molecular Biology
Hi everyone,i'm looking for a good southern blot protocol with all the secre
2024-10-09 15 -
Analysis of Genomic DNA by Southern Hybridization
Analysis of Genomic DNA by Southern HybridizationSelect several independent BAC
2024-10-09 15 -
Southern Blot using Neutral Transfer
-Elaine Pinheiro and Aurora Burds ConnorMIT, Jan2006This protocol works very wel
2024-10-09 14 -
采用优化红球菌的地高辛标记探针的DNA印迹方案（图）
采用地高辛高效标记混合物(ROCHE) 标记DNA探针将10 ng~3μg的DNA探针（基因组、质粒或基因片段）用蒸馏水稀释至16μl煮沸10分钟使DNA变性，
2024-10-09 13 -
Analys of Genomic DNA by Southe
Analys of Genomic DNA by Southern Hybridization (Southern Blot)Outline:Localizat
2024-10-09 14 -
In vitro transcription with yeast nuclear extract
Steve HahnLast Modified Fri,Apr 25,2003Wear gloves throughout,use RNAse free sol
2024-10-09 14 -
Overgo Probing of High-Density Filters
I have been using double-stranded 36mers form STS to probe high-density PAC/BAC
2024-10-09 15 -
EMSA using ds Oligonucleotides
Solutions 10X Annealing Buffer 200 mM Tris 8.0 200 m l 1M Tris pH 8.0 10 mM EDTA
2024-10-09 13 -
DNA labeling by nick translation
reagents: DNA for labeling (concentration c > 150 ng/µl) modified nucleotides
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