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  • "BEST" PCR--从质粒上扩增DNA的PCR条件

    "BEST" PCR--从质粒上扩增DNA的PCR条件

    BEST" PCR conditions for amplifying DNA from plasmids25 ng linear template

    2024-11-19 33
  • THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR

    THE FOUNDATION OF SUCCESSFUL RT IN SITU PCR

    TABLE OF CONTENTS 1. Abstract 2. Introductory statement 3. The key preparatory s

    2024-11-19 42
  • Protocol for Enhancing PCR of Very Difficult Regions

    Protocol for Enhancing PCR of Very Difficult Regions

    Protocol for Enhancing PCR of Very Difficult Regions Ziyun Yao, Shaoping Lin, Ho

    2024-11-19 54
  • Quantification of PCR using delta-delta Ct method.-Molec

    Quantification of PCR using delta-delta Ct method.-Molec

    One of the most used quantification methods in PCR is the "delta-delta Ct&q

    2024-11-19 55
  • is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea

    is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea

    The gene we are interested in looking at is not very abundant. At 100 ng/ul, the

    2024-11-19 49
  • SDS-PAGE--聚丙烯凝胶电泳

    SDS-PAGE--聚丙烯凝胶电泳

    DS-PAGE: gel electrophoresis of proteins TECHNIQUE is to set up gel plates befor

    2024-11-19 62
  • General PCR methods

    General PCR methods

    Polymerase Chain Reaction (PCR)(adapted from Bruce A. Roe, Department of Chemist

    2024-11-19 52
  • real-time PCR-Molecular Biology

    real-time PCR-Molecular Biology

    hi,I am going to do real-time PCR using Smart Cycler, but I have neverdone this

    2024-11-19 42
  • Long-PCR Reagents and Guidelines

    Long-PCR Reagents and Guidelines

    Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al.

    2024-11-19 52
  • Isolation of Retroelement from Plant Genomic DNA

    Isolation of Retroelement from Plant Genomic DNA

    The amplification of DNA fragments using the polymerase chain reaction (33) is

    2024-11-19 47
  • PCR基本实验方法(三)

    PCR基本实验方法(三)

    "Hot Start" PCR: In certain circumstances one wishes to avoid mixing p

    2024-11-19 53
  • Polymerase Chain Reaction

    Polymerase Chain Reaction

    Materials:Taq (or other) PCR enzyme (5 Units/ul)   PCR enzyme reaction buffer  

    2024-11-19 48
  • PCR principles and practices

    PCR principles and practices

    Important parameters in the PCR:1.Template DNA quantity (complexity determines n

    2024-11-19 46
  • PCR基本实验方法(四)

    PCR基本实验方法(四)

    Labelling PCR Products with DigoxigeninPCR products may be very conveniently lab

    2024-11-19 47
  • Primer4.10中文使用说明

    Primer4.10中文使用说明

    Primer4.10中文使用说明Primer Premier4.0是由加拿大的Premier公司开发的专业用于PCR或测序引物以及杂交探针的设计,评估的软件,和

    2024-11-19 49