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TRIzol法同时提取RNA、DNA、蛋白质
TRIzol试剂适用于从细胞和组织中快速分离RNA。TRIzol试剂有多组分分离作用，与其他方法如硫氰酸胍/酚法、酚/SDS法、盐酸胍法、硫氰酸胍法等相比，最大
2024-10-11 26 -
关于全血的保存-DNA提取-pcr个人经验
做了好多DNA提取保存，吃了很多亏给大家点建议吧：（有疑问可以回帖，有时间我会回答如果我知道）。 血液保存： EDTA抗凝最好，肝素也可以不过经常会影响后续
2024-10-11 21 -
乙醇沉淀DNA实验操作方法
1. 加入 1/10 体积的乙酸钠（3 mol/L ，PH=5.2）于 DNA 溶液中充分混匀，使其 最终浓度为 0.3 mol/L； 2. 加入 2 倍体积
2024-10-11 33 -
DNA抽提指南(TRIZOL)
按照RNA 分离操作方案在完全移去水样层后，匀浆中的DNA 存在于中间层和苯酚层中也可以被分离出来。在沉淀和多次洗脱后，DNA 溶解在8 mM NaOH中。用
2024-10-11 17 -
Chemical Sequencing of DNA
This is a rapid method for chemical DNA sequencing which is commonly used as lad
2024-10-11 13 -
PREPARATION OF SEQUENCING GELS
MATERIALS:2-glass plates1 sharks -tooth comb and spacersWhatman 3 mm paper30 or
2024-10-11 14 -
DNA SEQUENCING REACTIONS
DNA priming reactionx ul DNA 2 ul Reaction buffer, minus DTT 1 ul prim
2024-10-11 14 -
NO "WEDGE" Sequencing Gels
I have been using an alternative to wedge gels that saves acrylamide, cuts gel d
2024-10-11 12 -
Chemical Sequencing of DNA
This is a rapid method for chemical DNA sequencing which is commonly used as lad
2024-10-11 12 -
DNA Sequencing Gels
Buffers and gel solutions Long Ranger: we started using this in early 1995. Grea
2024-10-11 10 -
SEQUENCING GELS
10X TBE, per liter Sequencing dye ------------------- ----------
2024-10-11 12 -
Plasmid Sequencing
This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Pr
2024-10-11 12 -
DNA Sequencing Gels
Buffers and gel solutions Long Ranger: we started using this in early 1995. Grea
2024-10-11 13 -
DNA内电子传递
ＤＮＡ的双螺旋结构中碱基对在整个大分子链形成一个大的pai键 这个结构在导电高分子中很常见，这个键就是导电高分子导电的核心关键，而ＤＮＡ中的这个大pai键的作用
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DNA SEQUENCING (SANGER)
1. For double-stranded DNA templates:a. Denature template: 10ml DNA (5-10g or al
2024-10-11 12
