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mRNA差别显示技术
mRNA差别显示技术也称为差示反转录PCR(Differential Display of reverse Transcriptional PCR)简称为ddR
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长距离PCR
Long PCR (Church Lab) PCR conditioning for different templates, primer design,
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DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity
Andrea J. Gossett and Jason D. Lieb 1Department of Biology, University of No
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Reverse Ligation Mediated RT - PCR
This procedure was first described by Bertrand et al (Proceedings of the Nationa
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Singel Nucleotide Primer Extension (SNuPE)
Singel Nucleotide Primer Extension (SNuPE) Contributed by Dr. A. GratchevSingle
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Methylation-Specific PCR
Methylation-Specific PCRProtocol written by James Herman*Methylation-specific PC
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Thermal Cycling Profile for Standard PCR
Initial denaturationIt is very important to denature the template DNA completely
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质粒的Midi制备
Plasmid Midi-preps (NWSFC)Diatomaceous Earth-based midi-prep. This procedure
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TAIL PCR Protocol
TAIL is a series of reactions that are intended to map where a T-DNA (transfer D
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Colony PCR Protocol
1. Pull out eight glycerol stock plates from the �80oC freezer and set on bench
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原位聚合酶链式反应(in situ PCR)和原位反转录聚合酶链式反应(in situ RT-PCR)操作规程
(一)、仪器设备 英国 Thermo Hybaid 原位 PCR 仪。 (二)、操作流程 1 、原位 PCR 步骤 1 )
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分子实验方法8:聚合酶链式反应(PCR)扩增和扩增产物克隆
第八章 聚合酶链式反应(PCR)扩增和扩增产物克隆 第一节 概 述 PCR(Polymerase Chain Reaction,聚合酶链反应)是一种选择性体外
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PCR污染及解决对策
聚合酶链反应(polymerase chain reaction, PCR)是一种选择性体外扩增DNA或RNA片段的方法,在微生物感染的诊断中具有重要的价值。
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Primer Premier 引物设计
Primer Premier4.0是由加拿大的Premier公司开发的专业用于PCR或测序引物以及杂交探针的设计,评估的软件,和Plasmid Premier2
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逆转录-聚合酶链反应
逆转录-聚合酶链反应 (Reverse Transcription-Polymerase Chain Reaction,RT-PCR)的原理是:提取组织或细胞中
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