-
Is there a PCR length limitation after Bisulfite treatme
Hey,I am analyzing the methylation status of two regions: one ecompasses 500bp,
2024-10-10 17 -
Alternatives to demethylate DNA in vivo other than 5-aza
I'm looking for a methode to demethyle DNA in vivo , during more than 5 days
2024-10-10 20 -
Enzyme digestion before bisulfite treatment-DNA Methylat
HiDoes enzyme digestion before bisulfite treatment make a big difference - i am
2024-10-10 13 -
CpG island identification-DNA Methylation, Histone and C
Hi,I looked for CpG islands within the promoter of my gene.MethPrimer and CpG pl
2024-10-10 14 -
In situ immunodetection of 5-methylc
In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-
2024-10-10 19 -
About aza-cytidine-DNA Methylation, Histone and C
Hi,I'm starting to work whit 5' cytidine on plants.I want to know if it&
2024-10-10 14 -
CpG Island length variation-DNA Methylation, Histone and
CpG islands can be variable in length. Anyone know by what percentage range they
2024-10-10 18 -
Footprinting
FootprintingFootprinting is a method for determining the exact DNA sequence to w
2024-10-10 17 -
How short can be the CpG island-Molecular Biology
Hi there,Is anyone know how short of the CpG island can be? let say if the CG se
2024-10-10 15 -
Bisulfite modification kits and PCR-DNA Methylation, His
I was wondering if anyone could provide an opinion on the difference in PCR ampl
2024-10-10 18 -
DNA methylation
DNA methylation is an epigenetic event that affects cell function by altering ge
2024-10-10 11 -
Bisulfite Modification of DNA
Source: Protocol Online Abstract: Modifying DNA using sodium bisulfite to conver
2024-10-10 19 -
多囊卵巢综合征病人基因组DNA甲基化免疫沉淀反应分析
多囊卵巢综合征(Polycystic ovary syndrome, PCOS)是一种复杂的、病因不明的疾病，最新研究结果表明，胰岛素耐受(insulin re
2024-10-10 15 -
Problems in bisulfite sequencing reproducibility-DNA Met
I am having huge problems with bisulfite PCR.Southern analysis indicates that my
2024-10-10 16 -
甲基化 MSP 特异性 PCR 全程的常见问题与相关控制措施
一、亚硫酸氢钠修饰过程中可能出现的问题及应对措施亚硫酸氢钠修饰DNA的目的是将DNA序列中未甲基化的胞嘧啶完全转化为尿嘧啶，而甲基化的5一甲基胞嘧啶保持不变。影
2024-10-10 18
