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  • 质粒

    质粒

    Extrachromosomal Elements-Plasmids (Michael Blaber) What's plasmid? Find ans

    2024-11-13 21
  • PCR添加物

    PCR添加物

    PCR Additives (Robert H. Cruickshank)A usr/localiety of PCR additives and enha

    2024-11-13 15
  • SYBRGreen Q-PCR in the ABI 7700

    SYBRGreen Q-PCR in the ABI 7700

    Materials ROX Passive Reference Dye 25 µM, 50x (Invitrogen, Cat. No. 12223-012 )

    2024-11-13 31
  • 多重PCR反应中关键因素和实验步骤

    多重PCR反应中关键因素和实验步骤

    多重 PCR反应循环条件的优化延伸温度(步骤 5 , A-C ) . Figure 2c 展示了当加入相等量 (0.4 μM each) 的四种用于扩增 Y 染

    2024-11-13 37
  • PfuDNA聚合酶PCR实验方法介绍

    PfuDNA聚合酶PCR实验方法介绍

    General AdvicePCR allows the production of more than 10 million copies of a targ

    2024-11-13 16
  • Mouse p27 PCR Using Gitschier Buffer

    Mouse p27 PCR Using Gitschier Buffer

    Mutant allele: Neo-1 primer (CCTTCTATCGCCTTCTTG), plus mgK-3 primer (TGGAACCCTGT

    2024-11-13 24
  • mRNA差别显示技术

    mRNA差别显示技术

    mRNA差别显示技术也称为差示反转录PCR(Differential Display of reverse Transcriptional PCR)简称为ddR

    2024-11-13 28
  • 长距离PCR

    长距离PCR

    Long PCR (Church Lab) PCR conditioning for different templates, primer design,

    2024-11-13 22
  • DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity

    DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity

    Andrea J. Gossett and Jason D. Lieb 1Department of Biology, University of No

    2024-11-13 18
  • Reverse Ligation Mediated RT - PCR

    Reverse Ligation Mediated RT - PCR

    This procedure was first described by Bertrand et al (Proceedings of the Nationa

    2024-11-13 21
  • Singel Nucleotide Primer Extension (SNuPE)

    Singel Nucleotide Primer Extension (SNuPE)

    Singel Nucleotide Primer Extension (SNuPE) Contributed by Dr. A. GratchevSingle

    2024-11-13 22
  • Methylation-Specific PCR

    Methylation-Specific PCR

    Methylation-Specific PCRProtocol written by James Herman*Methylation-specific PC

    2024-11-13 21
  • Thermal Cycling Profile for Standard PCR

    Thermal Cycling Profile for Standard PCR

    Initial denaturationIt is very important to denature the template DNA completely

    2024-11-13 22
  • 质粒的Midi制备

    质粒的Midi制备

    Plasmid Midi-preps (NWSFC)Diatomaceous Earth-based midi-prep. This procedure

    2024-11-13 22
  • TAIL PCR Protocol

    TAIL PCR Protocol

    TAIL is a series of reactions that are intended to map where a T-DNA (transfer D

    2024-11-13 22