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质粒
Extrachromosomal Elements-Plasmids (Michael Blaber) What's plasmid? Find ans
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PCR添加物
PCR Additives (Robert H. Cruickshank)A usr/localiety of PCR additives and enha
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SYBRGreen Q-PCR in the ABI 7700
Materials ROX Passive Reference Dye 25 µM, 50x (Invitrogen, Cat. No. 12223-012 )
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多重PCR反应中关键因素和实验步骤
多重 PCR反应循环条件的优化延伸温度(步骤 5 , A-C ) . Figure 2c 展示了当加入相等量 (0.4 μM each) 的四种用于扩增 Y 染
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PfuDNA聚合酶PCR实验方法介绍
General AdvicePCR allows the production of more than 10 million copies of a targ
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Mouse p27 PCR Using Gitschier Buffer
Mutant allele: Neo-1 primer (CCTTCTATCGCCTTCTTG), plus mgK-3 primer (TGGAACCCTGT
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mRNA差别显示技术
mRNA差别显示技术也称为差示反转录PCR(Differential Display of reverse Transcriptional PCR)简称为ddR
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长距离PCR
Long PCR (Church Lab) PCR conditioning for different templates, primer design,
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DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity
Andrea J. Gossett and Jason D. Lieb 1Department of Biology, University of No
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Reverse Ligation Mediated RT - PCR
This procedure was first described by Bertrand et al (Proceedings of the Nationa
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Singel Nucleotide Primer Extension (SNuPE)
Singel Nucleotide Primer Extension (SNuPE) Contributed by Dr. A. GratchevSingle
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Methylation-Specific PCR
Methylation-Specific PCRProtocol written by James Herman*Methylation-specific PC
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Thermal Cycling Profile for Standard PCR
Initial denaturationIt is very important to denature the template DNA completely
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质粒的Midi制备
Plasmid Midi-preps (NWSFC)Diatomaceous Earth-based midi-prep. This procedure
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TAIL PCR Protocol
TAIL is a series of reactions that are intended to map where a T-DNA (transfer D
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