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  • Safety Aspects of Cell Culture

    Safety Aspects of Cell Culture

    1.Risk AssessmentThe main aim of risk assessment is to prevent injury, protect p

    2024-12-09 70
  • 细胞培养基的应用选择

    细胞培养基的应用选择

    选择培养基没有一定的标准,有几点建议可供参考:(1 )建立某种细胞株所用的培养基应该是培养这种细胞首选的培养基。可以查阅参考文献,或在购买细胞株时咨询。(2)

    2024-12-09 77
  • Osteoblast Harvest Adult Mice

    Osteoblast Harvest Adult Mice

    MaterialInstruments Solutions1 Curve Scissor 50 ml tube 70% Et

    2024-12-09 69
  • Abbreviations

    Abbreviations

    ACDP Advisory Committee on Dangerous PathogensBSA Bovine Serum albuminBSE Bovine

    2024-12-09 63
  • 胶质细胞培养(astrocyte, oligodendrocyte)

    胶质细胞培养(astrocyte, oligodendrocyte)

    取材及胶质细胞的混合培养1、P2 SD大鼠经低温麻醉后以碘酒和75%乙醇消毒,无菌操作下,断头放入预冷的D-Hanks液中,在解剖显微镜下取大脑皮层并除去脑膜。

    2024-12-09 177
  • 动物淋巴管内皮细胞的分离

    动物淋巴管内皮细胞的分离

    基本方案1:灌注消化法实验材料:1. 哺乳动物胸导管淋巴管2. 1PBS,含青霉素100u/ml和链霉素100μg/ml,pH7.43. 0.2%Ⅰ型胶原酶4.

    2024-12-09 99
  • 细胞培养中的常见污染

    细胞培养中的常见污染

    1、BacteriaBacteria(cocci) at 320x magnification. In this example, the cells are

    2024-12-09 121
  • Bcap-37和MDA-MB-435细胞的传代

    Bcap-37和MDA-MB-435细胞的传代

    Bcap-37和MDA-MB-435细胞都是人乳腺癌细胞,培养液用DMEM或1640均可,小牛血清10%就足够,不过感觉435细胞用DMEM长得更旺一些。两种细

    2024-12-09 74
  • Bel-7402 人肝癌细胞系

    Bel-7402 人肝癌细胞系

    AbstractAIM:To study the effect of a varying concentrations of ars enic trioxide

    2024-12-09 69
  • vero(非洲绿猴肾细胞)的传代

    vero(非洲绿猴肾细胞)的传代

    传代步骤比较传统:倒掉培养液->PBS洗2遍->胰酶0.25%消化->倒掉胰酶->加培养基->用吸管吹打均匀->分瓶->

    2024-12-09 64
  • 8 年细胞培养大神的经验指南!

    8 年细胞培养大神的经验指南!

    我养细胞有 8 年历史了,现在我的细胞已成为大家参观和崇拜、学习的对象。好的经验要分享,这就将我这几年来养细胞的经历和感想跟大家交流一下。启蒙老师的重要性一般进

    2024-12-09 75
  • Protocol for Cell Fusion

    Protocol for Cell Fusion

    Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should

    2024-12-09 57
  • 正常动物肠肥大细胞的培养

    正常动物肠肥大细胞的培养

    实验材料:1. 正常动物的肠或者手术切除标本的正常肠组织;2. 不含Ca2+ 和Mg2+ 的1PBS,添加200000IU/L青霉素、200mg/L链霉素,pH

    2024-12-09 74
  • How to Make Competent Cells

    How to Make Competent Cells

    Step 1: Take an 100 mL aliquot of frozen cells from the -80oC and innoculate abo

    2024-12-09 72
  • ES / MEF cell culture and electroporation of targeting construct

    ES / MEF cell culture and electroporation of targeting construct

    Day 0One frozen vial of Murine Embryonic Fibroblasts (MEFs) is thawed quickly in

    2024-12-09 76