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E.coli Total RNA Labeling Protocol for Spotted Microarray
Note: Start with 20 mg of total RNA for each labeling reaction. All solutions th
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Hiro Hirai PRINS Protocol
Hiro Hirai 's PRINS ProtocolOverviewThe PRINS (Primed in situ ) technique us
2024-11-02 19 -
[Nature] RNAi研究专题
Cells utilize RNA interference (RNAi) to regulate protein expression in many con
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Primer Extension analysis of total yeast RNA
Primer Extension analysis of total yeast RNA1. Mix the following in a 1.5 ml epp
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Northern Blotting (Howell Lab)
RNA isolationHarvest cells when they are about 80-85% confluent. Stationary phas
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RNA干扰沉默癌基因
RNA interference silences cancer geneDavid Douglas Reuters Health Posting Date:
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Collection of Tissue for RNA Analysis
Handling RNA: Ribonucleases (RNases) are very stable and active enzymes that deg
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Decontamination and inhibition of ribonucleases
Decontamination and inhibition of ribonucleasesIt is common knowledge in most la
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用异硫氰酸胍和有机溶剂提取ＲＮＡ
收集细胞，离心5000r/min ，5分钟，弃上清，加入异硫氰酸胍变性液（异硫氰酸胍4mol/L；柠檬酸钠25mmol/L，Sarkosy10.5%,β-巯基乙
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用异硫氰酸胍和有机溶剂提取RNA
收集细胞，离心5000r/min ，5分钟，弃上清，加入异硫氰酸胍变性液（异硫氰酸胍4mol/L；柠檬酸钠25mmol/L，Sarkosy10.5%,β-巯基乙
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C.elegans TOTAL RNA PREP
1. Collected animals can be stored as frozen pellets at -70 degrees. Alternativ
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五种不同的制备siRNA方法的比较
比较项目化学合成体外转录RNase III降解dsRNAsiRNA 表达载体PCR 表达框材料需求21-mer RNA oligos(一对)29-mer DNA
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RNA干扰技术的新突破
来自一家名为“ＡＬＮＹＬＡＭ”的 生物 技术公司的研究人员在11日出版的《自然》杂志上报告说，他们通过转基因技术在ＲＮＡ（核糖核酸）干扰技术的研究上取得了突破，
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Northern杂交试验指导手册－模板DNA的制备
A．质粒DNA的小量提取 试剂及其配制 含AP的LB液体培养基 TE缓冲液：10mmol/L Tris-HCl (pH 8.0) 1mmol/L EDTA (p
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miRNA的特征、功能及识别方法等详解
什么是miRNA MicroRNAs （miRNAs） 是一种大小约21—23 个碱基的单链小分子RNA ，是由具有发夹结构的约70-90 个碱基大小的
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