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Northern transfer protocol
A. Formaldehyde agarose gel electrophoresisNote: Formaldehyde vapours are toxic
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Fluorescence In Situ Hybridization
PRE-TREATMENTFix the cells with Carnoy’s and drop them onto a glass slide Air dr
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Hiro Hirai BAC-FISH Protocol
Hiro Hirai 's BAC-FISH ProtocolOverviewThis protocol is a modification of th
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Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel
Abstract: This is the standard and complete Northern blotting protocol including
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Northern blot实验技术
一、经乙二醛和二甲基亚砜变性处理后进行的RNA电泳这一方法原于McMaster和Carmichael(1977)。 含有乙二醛－DMSO的凝胶比含有甲醛的凝胶更
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RNA Isolation Protocol from LCM Samples
1. Drop sample into 100 μl Lysis Solution and incubate for 30 min at 42CUsing sh
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Protocol of Northern blot
Protocol of Northern blot 质粒的转化和扩增 质粒的鉴定 目的基因片段的切割 3.1样品双酶切(175μl水解体系) DW 115μl
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Hiro Hirai PRINS Protocol
OverviewThe PRINS (Primed in situ) technique uses a specific primer, dNTPs with
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Preparation of Poly A+ RNA and Northern Analysis
Preparation of Poly A+ RNA and Northern Analysis Procedure (Pharmacia #27-9255-0
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Solublization of RNA in Formamide
Solublization of RNA in Formamidecontributed by James McCaughern-Carucci, Yale U
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RNAi起源与专有名词
RNAi起源首次发现dsRNA能够导致基因沉默的线索来源于线虫Caenorhabditis elegans的研究。>1995年，康乃尔大学的Su Guo博
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mRNA In Situ Hybridization with Nick-Translated Probes
mRNA In Situ Hybridization with Nick-Translated ProbesCells are fixed with fresh
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Concentrating poly(A)+ mRNA
Concentrating poly(A)+ mRNASource: Protocol OnlineAbstract: Simple precipitation
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Transfection of siRNA oligos to peritoneal macrophages
Use DeliverX Transfection from PanomicsModified protocol belowStep 1: Make comp
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Northern Blot: Glyoxal/DMSO
Northern Blot: Glyoxal/DMSOAdapted from Molecular Cloning (Maniatis), Current Pr
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