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植物RNA提取过程中难点的相应对策
植物和动物组织有很大的不同,主要是其多糖及酚类化合物含量很多,因与有必要注意如下几个方面.酚类化合物的干扰及对策： 许多植物组织特别是植物的果实（如苹果、樱桃、
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The Basics: RNase Control
To the average molecular biologist working with RNA, preventing, detecting and e
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Northern Blot实验方法
[仪器、试剂、材料] （一）仪器 恒温水浴箱，电泳仪，凝胶成像系统，真空转移仪，真空泵，UV 交联仪，杂交炉，恒温摇床，脱色摇床，漩涡振荡器，分光光度计，微量移
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In situ hybridization of yeast cells (RNA and Oligonucleotide probes)
I. General considerations:In situ hybridization of yeast cells is almost identic
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Fluorometric Quantitation of RNA or DNA
Materials DNA: Höchst 33258 (High range solution): (Invitrogen H1398 or Sigma B
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PREPARATION OF STOCK SOLUTIONS FOR FISH
PREPARATION OF STOCK SOLUTIONS FOR FISH note: use for all preparations deionised
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Digoxigenin-UTP RNA labeling
Digoxigenin-UTP RNA labelingDigoxigenin-UTP RNA labeling 1. Add the following to
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RNA实验操作中的一般要求
在所有RNA实验中，最关键的因素是分离得到全长的RNA。而实验失败的主要原因是核糖核酸酶（RNA酶）的污染。由于RNA酶广泛存在而稳定，一般反应不需要辅助因子。
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Northern杂交试验指导手册－RNA提取
方法一、改良异硫氰酸胍提取法 试剂及其配制 异硫氰酸胍变性液： 贮存液－在含484ml 水（经DEPC处理）, 17.6ml 0.75mol/L柠檬酸钠（pH7
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What is your Protocol for RNAi on Cell Cultures
6-Well Plates Bathing Prepare dsRNA suspended in water. We use ~500 bp dsRNA. Ad
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Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel
Northern Hybridization of RNA Fractionated by Agarose-formaldehyde GelAuthor: Zu
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Increasing Your RNA Recovery During Tissue or Cell Extraction
One of the most problematic steps in RNA isolation is the first step - thorough
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NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGEN
NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGENIN dUTPFor the use with
2024-11-02 23 -
地高辛标记cRNA探针实验方法
1．组织前处理在组织制片中以冷冻切片（厚10～30μm）最佳。切片贴在预先清洁，高温处理并涂以粘附剂载玻片上，先在37℃预干燥4h，然后置于37℃烤箱中过夜。经
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RNA干扰及其应用进展
孙德惠 才学鹏 常惠芸 独军政 （1.中国农业科学院兰州兽医研究所家畜疫病病原 生物 学国家重点实验室农业部畜禽病毒学重点开放实验室，甘肃兰州 730046；2
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