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DROSOPHILA RNA PREP
DROSOPHILA RNA PREP (Goodman Lab)Stock Solutions3M NaOAc, pH 5.2 with HAc (MW=82
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Lentiviral strategies for RNAi gene knockdown
IntroductionAmong the 30-40 thousand protein-encoding genes in the human genome,
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Southern Hybridazation
HybridizationMaterials Prehybridization Solution (10 - 50 mL). Mix by rotating
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RNA酶保护试验((RNase Protection Assay,RPA)简介
RNA酶保护试验((RNase Protection Assay,RPA)是通过液相杂交的方式,用反义RNA探针与样品杂交，以检测RNA表达的技术。 1。原理：
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推荐：RNAi技术在功能基因组中的应用
在功能基因组研究中，需要对特定基因进行功能丧失或降低突变，以确定其功能。由于RNAi具有高度的序列专一性，可以特异地使特定基因沉默，获得功能丧失或降低突变，因此
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Northern blotting - gel preparation and treatment(Northern杂交-凝胶制备及处理)
RNA is separated under denaturing conditions; the principal systems currently in
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RNA干扰在慢性疼痛治疗中的应用
第二军医大学东方肝胆外科医院麻醉科 吴飞翔 综述 俞卫锋 审校 摘要 慢性疼痛的治疗临床上效果不佳，集中发展更多有效的治疗方法已经成为一个普遍的主题。RNA干
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WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos
June, 1997 - optimized by N. Kertesz, L.Leyns & E. De RobertisPlease cite: Belo
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In situ hybridization of yeast cells (RNA and Oligonucleotide probes)
I. General considerations:In situ hybridization of yeast cells is almost identic
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siRNAs可以发挥miRNAs功能
Doench JG, Petersen CP, Sharp PA. (2003) siRNAs can function as miRNAs. Genes De
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Agarose-Formaldehyde Electrophoresis
Agarose-Formaldehyde ElectrophoresisRNA electrophoresis under denaturing conditi
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Protein-DNA Telomere FISH
Protein-DNA Telomere FISH FIX AND PROTEIN STRAIN:Fix cells in 4% paraformaldhyde
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Northern Blotting: Efficient RNA Staining and Transfer
(original protocol by R. M. Fourney, S. Miyakoshi, R. S. Day III, and M. C. Pete
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Pre-hyb/hyb for Radioactive Probes
Pre-hyb/hyb for Radioactive ProbesStuff you need:50X Denhardt's reagent:1% F
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RNAi-制备siRNAs的方法
越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链
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