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De-phosphorylation of DNA
The preferred method of de-phosphorylation uses the buffer system described by P
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Recoveryof DNA from LMP(Low Melting Point)AgaroseGel
Recovery of DNA from LMP (Low Melting Point) Agarose Gel1. Separate DNA fragment
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Gene-Kleen protocol
Purification of DNA from agarose gels is an essential method involved in the sub
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IsolationofDNAfromMouseTai
Isolation of DNA from Mouse Tail Biopsies1. Obtain tail biopsies from 2 to 3 wee
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PROTOCOL TO EXTRACT DNA FROM PA
PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS1.Cut 10-20X10μm sections of form
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CTABTECHNIQUE Method Schedule Protocol(JPB)FOR DNA
CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTR
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BUCCAL CELL DNA PREPS
Important : Extract the DNA within one week of receiving samples. Samples that h
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Isolation of DNA from Acrylamide GELS
1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is
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ProtocolforIsolationofGenomicDNAfromMammalianCells
Protocol for Isolation of Genomic DNA from Mammalian Cells1.Trypsinize, harvest
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DNAFRAGMENTPURIFICATIONW/GLASSWOOL
Glass wool method:Run TAE agarose gel and cut the appropriate band out with a cl
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SilicaClean-upofDNA
Materials:Silica Suspension:add 2 g of silica to 15 ml of H2 Owash 3x by centifu
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DNAPurificationbyphenolextractionandethanolprecipitation
Equipment and reagents1. Phenol2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM E
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Isolating DNA Fragments
Materials:• 0.8 % agarose gel in 1x TAE• Digested DNA• Glass Milk
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Target enrich mentte chnologies for ABISOLiD
Q:my group is involved in a national project to develop a genomic platform for c
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Ribonuclease Protection Assay
1. Add appropriate volumes of RNA (<12 ul) plus probe (< 3 ul). Try 5 and
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