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Self-circularization of Linear DNA
一、In a microcentrifuge tube prepare a solution of linear DNA (25-50ng) in deioni
2024-10-21 15 -
Electro-elutionofnucleicacidsfromagaroseandpolyacrylamidegelslides
PrincipleThe nucleic acids contained within the gel slice are electrophoresed ou
2024-10-21 14 -
DNAISOLATIONFROMPRIMARYTUMORSVIACRYOSECTIONS
- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sec
2024-10-21 15 -
Improved Alcohol Precipitation of DNA
ECKDescriptionThis method was suggested in a BRL "Focus" article sever
2024-10-21 15 -
DNA Fragment Purification from Agarose or Acrylamide
DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to
2024-10-21 17 -
Preparation of GA Marker
Author: Long-Cheng LiSource: Protocol OnlineAbstract: Simplified method for prep
2024-10-21 14 -
RecoveringDNAfromagarosegels
IntroductionMethods and reagents is a unique monthly column that highlights curr
2024-10-21 12 -
Boilinglysismini-prep
1、Grow an overnight culture from a single colony in 2 mls LB antibiotic.2、Transf
2024-10-21 16 -
TACloning
TA CloningI. Initial mixture5 μl dd H2O1 μl PCR product1 μl ligation bu
2024-10-21 17 -
NH4AcandEtOHprecipitationofDNA
Add NH4Ac (10 M stock or solid) to the sample for a final concentration of 2.5 M
2024-10-21 15 -
ElectroelutionofDNAfromAgarose
Electroelution of agarose fragmentsElectroelution buffer1 M Tris, pH 7.5 12.0 ml
2024-10-21 18 -
Phlebotomus Sand flies的DNA的提取
1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50
2024-10-21 24 -
BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
1、A smear (rather than a single colony) of BAC colonies are picked and transferr
2024-10-21 18 -
DNA Extraction Methods
Phenol-chloroform DNA extraction from sand flies1. Crash Individual sand flies i
2024-10-21 12 -
How to isolatesingle-stranded DNA frommammalian-Molec
Does anybody know how to isolate single-stranded DNA from mammalian cells?Thanks
2024-10-21 16
