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DNA Purification from Agarose Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide
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Oligo nucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
2024-10-21 12 -
Extraction from Cheek Cells DNA
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of an
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Preparation of TonsilLysate
PurposeThe protocol describes how to prepare human tonsil lysate for use in puri
2024-10-21 11 -
Oligo nucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
2024-10-21 15 -
Extraction from Cheek Cells DNA
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of an
2024-10-21 13 -
Preparation of plasmid DNA（质粒制备）
Preparation of plasmid DNA : a modified mini alkaline-lysis/PEG precipitation pr
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Analysis of Differential Gene Expression
Microarray analysis and differential display have become popular techniques for
2024-10-19 13 -
MINI PREP BY BOILING
Pellet 1.5 ml of an overnight culture at 12,000 rpm in Eppendorf centrifuge at R
2024-10-19 17 -
Phenol/Chloroform Precipitation of DNA
1. Add an equal volume (equal to sample volume) of P/C to sample.2. Mix (shake,
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核酸抽提mRNA的分离技巧
mRNA的分离 与rRNA和tRNA不同的是，哺乳动物细胞的绝大部分mRNA在其3'端均有一poly(A)尾，因此可以用oligo(dT)－纤维素亲和层
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DNA Purification and Precipitation
1.Prepare or obtain Buffered phenol,pH 8.0.Add 2 crystals of 8-Hydroxyquinoline
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DNAExtractionProtocolsUsingSilica
Below we present the protocols we have used to isolate DNA from various tissues
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CLONING FROM LOW MELT AGAROSE
competent cells (DH5alpha at competency of 108 cfu/µl of pUC18). The transformat
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Isolation of DNA from Agarose Gels(Paper Slurry Method)
Isolation of DNA from Agarose Gels (Paper Slurry Method)This procedure isolates
2024-10-19 9
