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科氏芽孢杆菌菌种
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一水草酸钙晶体刺激肾近端小管细胞后miRNA表达谱的变化
华中科技大学同济医学院泌尿外科研究所的研究人员证实,在一水草酸钙刺激肾近端小管细胞后,miRNA的表达谱发生了变化,这将暗示着miRNA也许在结石形成中发挥作用
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TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
TISSUE PREPARATION FOR IN SITU HYBRIDIZATION Josiah N. WilcoxHarvest tissue and
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RNA干扰技术在肿瘤研究中的应用
摘要: RNA 干扰(RNAi)是近几年发展起来的一种阻抑基因表达的新方法,它通过双链RNA的介导,特异性地阻断或降低相应基因的表达。目前,随着RNAi分子机制
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Spectrophotometric Analysis of rRNA
Spectrophotometric Analysis of rRNALEVEL IMaterials RNAUV spectrophotometer and
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LiCl RNA Preparation (Ambros Lab)
LiCl RNA Preparation (Ambros Lab)1) Freeze worms by dropping into liquid nitroge
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IN SITU HYBRIDIZATION ON FROZEN SECTIONS
IN SITU HYBRIDIZATION ON FROZEN SECTIONS Protocol by Josiah N. Wilcox, Ph.D.Remo
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The Neverfail Northern Blot Hybridization
The "Neverfail" Northern Blot Hybridization Contributed by James McCau
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Purification of poly(A)+ RNA
0. (Optional)Before using Oligotex-dT30, I recommend to wash Oligotex-dT30 to re
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35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION
35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION by Josiah N. Wilcox,
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Practical Tips for Handling RNA
Phenol ExtractionOne of the most common molecular biology lab procedures, phenol
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Phenol Extraction of rRNA (Rat liver)
Phenol Extraction of rRNA (Rat liver)LEVEL II *** READ THROUGH ALL CAUTIONS BEFO
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Water Treatment with DEPC
Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave
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Northern Blotting
Northern BlottingAdapted from Molecular Cloning (Maniatis), Current Protocols in
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Top Ten Ways to Ensure Valid RNAi Data
1.Confirm results with a second or third siRNA to the same target. One of the
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