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Northern protocol(RULES FOR RNA WORK)
1. Wear gloves at all time including filling pipet tip in racks, filling
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How to prepare Molecular Biology grade glycogen
How to prepare Molecular Biology grade glycogenAuthor: Roberto SalviSource: Cont
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Purification of poly(A)+ RNA from total RNA
Purification of poly(A)+ RNA from total RNA0. (Optional)Before using Oligotex-dT
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RNA interference：The short answer
One way of seeing what a gene does is to block its messenger RNA and note the ef
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RNAi——制备siRNAs的几种方法
越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链
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Preparation of single-stranded probes from cloned cDNA
a) 2-5 µg of plasmid DNA containing the cDNA insert is linearized using an appro
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IN SITU HYBRIDIZATION ON FROZEN SECTIONS
Remove slides from freezer, thaw for 5 min. at 55C. Fix 10 min. in 4% paraformal
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如何确定RNA质量的经验谈！
1） 检测RNA溶液的吸光度 280、320、230、260nm下的吸光度分别代表了核酸、背景（溶液浑浊度）、盐浓度和蛋白等有机物的值。一般的，我们只看OD26
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How to Maintain an RNase-free Lab
How to Maintain an RNase-free LabLet's admit it, everyone who has worked wit
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35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION
Pipet 12.5µl 35S-UTP (1200 Ci/mmol) into 1.5ml microfuge tube. Final concentrati
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S1 analysis of yeast mRNA using oligonucleotide probes
S1 analysis of yeast mRNA using oligonucleotide probesSteve Hahn. last modified
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推荐：RNAi及其在植物遗传改良中的应用
RNAi即RNA干涉(或RNA干扰)是指双链RNA导入细胞后并被切割成21～23个核苷酸长度的短核苷酸双链，在其它作用因子的参与下能够特异地与其同源的mRNA结
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哺乳动物RNAi技术全面实验方法
WHENVIRUSESINFECTEUKARYOTICCELLS domly integrate into host genomes, double-stran
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RNAi在首次动物实验中表现良好
研究人员首次利用包装入病毒载体中的小分子RNA对有遗传疾病的小鼠进行治疗。这一成果提高了RNA干扰技术能够运用在人类临床试验上的可能性。 亨丁顿舞蹈症（Hunt
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Injection DNA Preparation
Injection DNA Preparation1) Culture 400 mls of bacteria harboring transposon ove
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