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牵牛花与RNA干扰
RNAi现象的首次发现，是在外源的多拷贝转基因被引入牵牛花后所激起的一个特定序列的基因沉默响应。RNAi导致了封面上的花的双色彩图案。Ａｒｇｏｎａｕｔｅ是RNA
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PRINS步骤
1、常规脱蜡浸入0.01mol/LPBS； 2、用0.2mol/L盐酸处理5min； 3、蛋白酶K(25μg/m1)消化37℃ 15min
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互联网上的RNA/RNAi相关网址
一、Three-dimensional structures (coordinates and images): 1、 The Nucleic Acid Dat
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RNEasy Midi
Materials Qiagen RNEasy Midi Kit Dounce homogenizers (for tissues) Syringes and
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Preparation of probes for in situ hybridization
I. DNA oligonucleotide probes:DNA oligonucleotides are the preferable probes at
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Nucleotide Composition of RNA
Nucleotide Composition of RNALEVEL IIMaterials RNA sample1 N and 0.1 N HClBoilin
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RNA 操作注意事项与实验技巧
操作注意事项： 由于RNA酶的广泛存在与难以灭活的顽固特性。使得RNA的提取纯化和后续工作变得非常困难。为了保证RNA的研究工作成功，请仔细阅读下列注意事项
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TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
Harvest tissue and rinse in PBS or saline. Immerse tissue in 4% paraformaldehyde
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Northern Blotting (Breeden Lab)
Northern Blotting (Breeden Lab)关键词： northern blotting breeden lab来源： 互联网I. Elect
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Northern Blots
Northern Blotsby Michael Koelle and Tory Herman, adapted from Sambrook et al., &
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Northern杂交试验指导手册－RNA质量检测
二、RNA质量检测 提取的RNA需要对其质量和数量进行检测。实验室常用的方法有紫外吸收值检测和电泳检测两种。 方法一、紫外吸收检测 试剂：TE ( 10mmol
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Northern Blot
I. Electrophoresisclean gel box with NaOH and/or SDS, 2 hours to overnight, rin
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DROSOPHILA RNA PREP
DROSOPHILA RNA PREP (Goodman Lab)Stock Solutions3M NaOAc, pH 5.2 with HAc (MW=82
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Lentiviral strategies for RNAi gene knockdown
IntroductionAmong the 30-40 thousand protein-encoding genes in the human genome,
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Southern Hybridazation
HybridizationMaterials Prehybridization Solution (10 - 50 mL). Mix by rotating
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