-
Hiro Hirai BAC-FISH Protocol
Hiro Hirai 's BAC-FISH ProtocolOverviewThis protocol is a modification of th
2024-11-02 48 -
Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel
Abstract: This is the standard and complete Northern blotting protocol including
2024-11-02 40 -
Northern blot实验技术
一、经乙二醛和二甲基亚砜变性处理后进行的RNA电泳这一方法原于McMaster和Carmichael(1977)。 含有乙二醛－DMSO的凝胶比含有甲醛的凝胶更
2024-11-02 65 -
RNA Isolation Protocol from LCM Samples
1. Drop sample into 100 μl Lysis Solution and incubate for 30 min at 42CUsing sh
2024-11-02 45 -
Protocol of Northern blot
Protocol of Northern blot 质粒的转化和扩增 质粒的鉴定 目的基因片段的切割 3.1样品双酶切(175μl水解体系) DW 115μl
2024-11-02 66 -
Hiro Hirai PRINS Protocol
OverviewThe PRINS (Primed in situ) technique uses a specific primer, dNTPs with
2024-11-02 59 -
Preparation of Poly A+ RNA and Northern Analysis
Preparation of Poly A+ RNA and Northern Analysis Procedure (Pharmacia #27-9255-0
2024-11-02 48 -
Solublization of RNA in Formamide
Solublization of RNA in Formamidecontributed by James McCaughern-Carucci, Yale U
2024-11-02 50 -
RNAi起源与专有名词
RNAi起源首次发现dsRNA能够导致基因沉默的线索来源于线虫Caenorhabditis elegans的研究。>1995年，康乃尔大学的Su Guo博
2024-11-02 53 -
mRNA In Situ Hybridization with Nick-Translated Probes
mRNA In Situ Hybridization with Nick-Translated ProbesCells are fixed with fresh
2024-11-02 56 -
Concentrating poly(A)+ mRNA
Concentrating poly(A)+ mRNASource: Protocol OnlineAbstract: Simple precipitation
2024-11-02 55 -
Transfection of siRNA oligos to peritoneal macrophages
Use DeliverX Transfection from PanomicsModified protocol belowStep 1: Make comp
2024-11-02 42 -
Northern Blot: Glyoxal/DMSO
Northern Blot: Glyoxal/DMSOAdapted from Molecular Cloning (Maniatis), Current Pr
2024-11-02 54 -
植物RNA提取过程中难点的相应对策
植物和动物组织有很大的不同,主要是其多糖及酚类化合物含量很多,因与有必要注意如下几个方面.酚类化合物的干扰及对策： 许多植物组织特别是植物的果实（如苹果、樱桃、
2024-11-02 56 -
The Basics: RNase Control
To the average molecular biologist working with RNA, preventing, detecting and e
2024-11-02 59
