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Preparation of Affinity Column制备亲和层析柱【UCSF】
1.Add 222.2μl 1M MOPS,pH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final
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Crystallization Trials
MaterialsMilipore filter type HA 0.45 micronCulture plates (Linbro model 76-033-
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Preparation of Cell Lysate
1. Wash adherent cells twice in the dish or flask with ice-cold PBS and drain of
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Purification of recombinant sBRF M166L
J. Movius, K, Coachman, and S. Hahn (Hahn Lab)Induction of BRF in bacteria and p
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Preparation of Cell Lysate
1.Wash adherent cells twice in the dish or flask with ice-cold PBS and drain off
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Purification of dnEBNA-1/Soft from E. coli BL21 LysS
Inoculate 2ml of 5ml o/n culture of either p3133 (empty vector pET11a)or p3134 (
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Rapid Yeast Protein Prep for SDS PAGE and Western
Sample Buffer:10 ml0.06M Tris-HCl, pH 6.80.6 ml 1M Tris 6.810% (v/v) glycerol2 m
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Large scale nuclear extract preparat
Large scale nuclear extract preparat关键词： nuclear extract preparat来源： 互联网Hattoti&
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Purifying Protein from Inclusion Bodies
Buffer ABuffer B50 mM tris-HCl, pH 8.020 mM Na2 HPO4 , pH 7.25 mM EDTA20 mM NaCl
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Preparation of protein extracts for western blot
1.Grow cells to mid-log (OD600 less or equal to 1.0).2.Harvest about 5 OD's
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Urea Lysis Protocol
9M Urea,2.5mM EDTA,2.5mM EGTA,1% DTE,4% CHAPSmake 10ml and aliquot 10x1ml,freeze
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TCA protein precipitation
Kyle Coachman/Linda Warfield/Steve HahnLast modified 5/13/02To concentrate prote
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Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale)Hahn Lab (adapted from J.Leatherwood,Ptashne
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Purification of GST fusion proteins in E.coli GST融合蛋白纯化，方法二
GST Protein Prep.Ver.21)Grow 50ml of culture in LB or TB antibiotic o/n at 37℃ s
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Yeast protein prep for SDS PAGE and western (rapid)
Horvath and Riezman,Yeast,1994; Gottschling LabSample Buffer:10 ml0.06M Tris-HCl
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