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TCA protein precipitation
Kyle Coachman/Linda Warfield/Steve HahnLast modified 5/13/02To concentrate prote
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Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale)Hahn Lab (adapted from J.Leatherwood,Ptashne
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Purification of GST fusion proteins in E.coli GST融合蛋白纯化，方法二
GST Protein Prep.Ver.21)Grow 50ml of culture in LB or TB antibiotic o/n at 37℃ s
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Yeast protein prep for SDS PAGE and western (rapid)
Horvath and Riezman,Yeast,1994; Gottschling LabSample Buffer:10 ml0.06M Tris-HCl
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Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura
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Purification of Calmodulin from Bovine Brain
References: Watterson et al.(1980)J Biol Chem 255:962-75.Charbonneau et al.(1979
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离子交换层析介质的技术数据
关于离子交换层析实验的介绍，对于常用到的层析介质的一些基本技术数据罗列如下： 离子交换介质名称最高载量颗粒大小（μm ）特性/ 应用pH 稳定性工作耐压（MPa
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Isolation of Protein from Tissue
Place the tissues on labeled aluminum foil and immediately place in dry ice.It i
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Purification of Myosin Light Chain Kinase
Solutions1.1 M Acetic Acid2.Buffer A Homogenization 10 mM MOPS- 40 ml of 2M stoc
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Bacterial Protein Extraction (mini-scale)
Bacterial Protein Extraction (mini-scale)Using B-PerB-Per (Pierce,Cat number: 78
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Fusion Protein Isolation 融合蛋白分离纯化
1.Start a 5 ml overnight culture from a frozen stock (in the -70℃)in the followi
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Preparation of Tonsil Lysate
PurpseThe prtcl describes hw t prepare human tnsil lysate fr use in purificatin
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rat liver cytosol preperation
These protocols should yield enough cytosol and organelles for 1-200 MT/Organell
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HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1 liter cell prep1)g
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Yeast mini whole cell extract using glass beads for western analysis
Before trying this method,try rapid protein prep which involves boiling yeast in
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