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MicroRNA Northern Protocol
Gel Setup Using Protean II systemClean materials with 10% SDS and rinse thorough
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c—myc靶向小干扰RNA诱导乳腺癌细胞
c—myc靶向小干扰RNA诱导乳腺癌细胞关键词: 靶向 干扰 rna 诱导来源: 互联网c—myc靶向小干扰RNA诱导乳腺癌细胞翟荣林王国斌 夏泽峰 【摘要】
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MicroRNA和siRNA克隆规程(MicroRNA and siRNA Cloning Protocol)
RNA Cloning Method Flowchart Extract total RNA containing small RNAs. Check qual
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Non-fluorescent protein/RNA double-labeling
1. Collect embryos, dechorionate and fix in a (1:1) mixture of heptane:fix. Fix
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Multiple Tissue Northern(MTN)Blots
人、小鼠和大鼠的多种组织来源的高质量mRNA,经电泳分离后预转于尼龙膜上预制的即用型Northern杂交膜,免去RNA电泳操作过程可检测范围:0.5-10kb可
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A Detailed Procedure for RNase Activity Staining on SDS-PAGE
A Detailed Procedure for RNase Activity Staining on SDS-PAGE 1. Remove glass pl
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RNA in situ protocol for DNA and RNA probes
RNA in situ protocol for DNA and RNA probesThis procedure was adapted from one o
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Bulge-LoopTM miRNAs qRT-PCR Primer Set使用说明
锐博生物是国内最专业的miRNA研究服务公司,可提供miRNA研究的全套相关产品和服务,包括对细胞、血液、组织及其它体液中miRNA的高特异、高灵敏度检测引物和
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MAPPING TRANSCRIPTION INITIATION SITES WITH PRIMER EXTENSION
The primer extension re action is used to determine the start site(s) of RNA tra
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RNA电泳实验方法
Polyacrylamide Gel Electrophoresis (PAGE)for use withRibonuclease Protection Ass
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In vitro transcription with yeast nuclear extract
In vitro transcription with yeast nuclear extractSteve HahnLast Modified Fri, Ap
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DNA芯片检测siRNA专一性
长片断的双链RNA (dsRNA) 导入例如植物,真菌,果蝇,线虫等 生物 的细胞中会引发同源mRNA的降解——这就是所谓的RNA interference (
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TRIzol Prep
Procedure 1. Homogenize cells (10 million) or tissue (50-100 mg) in 1 mL TRIzol
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植物病毒的RNA提取
大多植物病毒RNA为单链RNA,并且其极性与mRNA极性相同,植物病毒RNA提取较为简单,一般使用酚氯仿即可获得满意结果。 一、材料 提纯TMV病毒液(10mg
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用RNAi研究基因组印迹现象的调控机制
HHMI News-January 09,2004-New Insight into Control of Parental Gene Expression i
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