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ES CELL DNA EXTRACTION: TUBE ME
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but p
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DNA From Whole Blood for PCR
1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapil
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Plant Total DNA Isolation
OverviewProcedure1. Preheat the CTAB Isolation Buffer at 60℃.2. Grind 2 g of fre
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Southern Blot Protocol
Day 11. Digest DNA for 6 hours (or overnight)BSA 10 mg/ml 0.5 22.65 ul of 10 ug
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Largescale nuclear extract preparation
Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Kari
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UV Quantitation of DNA
DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus
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DNAPrecipitation
Phenol (removes protein)1.add equal volume of Phenol (= tris-saturated Phenol-Ch
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Protocols for ET recombination
1、Oligo design（1）The 5' end (the homology arm) - choose 42 or more (we usual
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Isopropanol precipitation-best time and temperature-M
Hi, does anyone know what is the best temperature and time for Isoprop precipita
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DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS
- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sec
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Preparation of BAC DNA
The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB wi
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SimplifiedDNAExtractionfromCellorTissue
PurposeDNA extraction without phenol extraction and centrifugation.Procedure1. L
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Tg 008 Southern Blotting
Transgenic Mouse and Gene Targeting FacilityTg 008 Southern BlottingMaterials an
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DNA Purification from Agarose Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide
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Oligo nucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
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