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Membrane Transfer and Crosslinking for RNA
Analysis of mRNA expression in tissue or cell culture is often done by Northern
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Silica Clean-up of DNA
Materials:Silica Suspension:add 2 g of silica to 15 ml of H2Owash 3x by centifug
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Electroelution of DNA from Agarose
Electroelution of agarose fragmentsElectroelution buffer1 M Tris, pH 7.5 12.0 ml
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silica回收DNA片段
1、在长波紫外灯下切下含有目标DNA的琼脂糖凝胶， 用纸巾吸干凝胶表面的液体并切小。计算凝胶的重量。2、加入2-3倍体积的6M KI或NaI（避光4度保存）。3
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CsCl Prep of Plasmid DNA
This is a standard large scale prep.for plasmid DNA which gives a yield of 0.5-1
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Salmon Sperm DNA (10mg/ml)
Salmon Sperm DNA1.Dissolve 1 g salmon sperm DNA in 100 ml H2 O. 2.Autoclave (20
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详细BAC DNA Isolation
A. Culture Growth:1.For BAC isolation for shotgun library construction:Pick a sm
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Simplified DNA Extraction from Cell or Tissue
PurposeDNA extraction without phenol extraction and centrifugation.Procedure1. L
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DNA Recovery With Low Melt Agarose
Recovery of DNA from Low Melting Point Agarose Gels1.Run digestion products on 0
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Preparation of BAC DNA with by Alkaline Prep Method
CLONE ISOLATION PREP - PROTOCOLBefore you begin isolating DNA from the cultures:
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Yeast Nuclear Extract (Small Scale)
Hahn Lab (adapted from J.Leatherwood,Ptashne lab)last modified Mon,Aug 27,2001Ce
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RNAi Glossary
Argonaute - A family of proteins containing multiple domains and involved in RNA
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MinElute DNA纯化
做分子生物学实验，核酸纯化、酶切、克隆算是最基本的步骤了。因为要做定向克隆，通常要作双酶切。为了省时省事儿，我们常常想方设法把两个酶放在一起切。可是事情并非总
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植物基因组的快速提取
Use from 0.01 - 0.1 gram plant material.Grind the plant material with liq. N2 in
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琼脂糖凝胶回收DNA――纸浆法
This procedure isolates DNA from agarose gels by filtration through a filter-pap
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