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亚硫酸氢盐修饰后测序法检测甲基化——DNA甲基化
法原理重亚硫酸盐使DNA中未发生甲基化的胞嘧啶脱氨基转变成尿嘧啶，而甲基化的胞嘧啶保持不变，用PCR扩增（引物设计时尽量避免有CpG，以免受甲基化因素的影响）所
2024-10-10 52 -
DNA 亚硫酸氢盐修饰和纯化操作步骤
DNA 亚硫酸氢盐修饰和纯化操作步骤修饰设计：使用 CpGenomeTMkit 使胞嘧啶转化为尿嘧啶的步骤如下。中等温度碱性pH 下使 DNA 变性成为单链形式
2024-10-10 38 -
Method to look for novel methylated gene-DNA Methylation
Hi. I am a new member of this forum. Currently I am trying to look for some nove
2024-10-10 40 -
Methylation analyis using restriction
Methylation analyis using restriction enzyme digestionThis method has some weak
2024-10-10 34 -
Is there a PCR length limitation after Bisulfite treatme
Hey,I am analyzing the methylation status of two regions: one ecompasses 500bp,
2024-10-10 28 -
Alternatives to demethylate DNA in vivo other than 5-aza
I'm looking for a methode to demethyle DNA in vivo , during more than 5 days
2024-10-10 32 -
Enzyme digestion before bisulfite treatment-DNA Methylat
HiDoes enzyme digestion before bisulfite treatment make a big difference - i am
2024-10-10 22 -
CpG island identification-DNA Methylation, Histone and C
Hi,I looked for CpG islands within the promoter of my gene.MethPrimer and CpG pl
2024-10-10 24 -
In situ immunodetection of 5-methylc
In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-
2024-10-10 33 -
About aza-cytidine-DNA Methylation, Histone and C
Hi,I'm starting to work whit 5' cytidine on plants.I want to know if it&
2024-10-10 25 -
CpG Island length variation-DNA Methylation, Histone and
CpG islands can be variable in length. Anyone know by what percentage range they
2024-10-10 28 -
Footprinting
FootprintingFootprinting is a method for determining the exact DNA sequence to w
2024-10-10 25 -
How short can be the CpG island-Molecular Biology
Hi there,Is anyone know how short of the CpG island can be? let say if the CG se
2024-10-10 28 -
Bisulfite modification kits and PCR-DNA Methylation, His
I was wondering if anyone could provide an opinion on the difference in PCR ampl
2024-10-10 32 -
DNA methylation
DNA methylation is an epigenetic event that affects cell function by altering ge
2024-10-10 21
