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高通量、低成本SNP、突变或甲基化检测方法—HRM 技术应用
HRM 介绍HRM 技术是high-resolution melting analysis 即高分辨熔解曲线分析技术，是近年国外兴起的一种全新的突变扫描和基因分
2024-10-10 45 -
焦磷酸测序在表观遗传学中的应用
焦磷酸测序在表观遗传学中的应用—预先设计的反应体系可进行对人类全基因组DNA甲基化进行可靠检测和准确定量 在表观遗传学的DNA甲基化研究中，新一代的测序方法
2024-10-10 42 -
表观遗传学修饰
组蛋白修饰 表观遗传学是指表观遗传学改变 (DNA 甲基化、组蛋白修饰和非编码 RNA 如 miRNA) 对 表观基因组基因表达的调节，这种调节不依赖基因序
2024-10-10 49 -
DNA甲基化研究方法的回顾与评价
摘要： DNA甲基化是表观遗传学(Epigenetics)的重要组成部分，在维持正常细胞功能、遗传印记、胚胎发育以及人类肿瘤发生中起着重要作用，是目前新的研究热
2024-10-10 48 -
Southern Hybridization Protocols
Southern Hybridization ProtocolsTwo protocols are given here.The first one is us
2024-10-09 41 -
Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisatio
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Labeling oligonucleotides with 32P ATP
Wear gloves throughout and work in radiation area. Monitor area before and after
2024-10-09 34 -
Genomic DNA Labeling Protocol
We typically use 0.5ug of E. coli genomic DNA in a labeling reaction for each hy
2024-10-09 28 -
Removal of 32P-ATP from Oligonucleoi
1. Prepare Whatman DE-52 according to manufacturers specifications. Equilibrate
2024-10-09 36 -
Labeling oligonucleotides with 32P ATP
Wear gloves throughout and work in radiation area.Monitor area before and after
2024-10-09 30 -
Fill-in Labeling of DNA Fragments
This protocol was designed to generate directionally end-labeled probes for DNas
2024-10-09 26 -
Screen ES cells by Southern Blot
Digest DNA in 96-well plateTo each well add:4μl 10Xbuffer4μl Enzyme0.4μl Spermid
2024-10-09 24 -
Tail Chop Southern Protocol
About 1/2 to 1 cm of tail should be cut from properly marked mice (using toe or
2024-10-09 23 -
SSC-Molecular Biology
Hi,Can you tell me what do "SSC" in a southern blot, how he act on DNA
2024-10-09 26 -
Southern Blotting(Fred Hutchinson Cancer Research Center)
DNA Transfer1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (sma
2024-10-09 28
